Glomerular thromboxane A2/prostaglandin H2 receptors: characterization and effect of adriamycin-induced nephrotic syndrome.
Glomerular thromboxane A2/prostaglandin H2 receptors: characterization and effect of adriamycin-induced nephrotic syndrome.
复制标题
肾小球血栓素 A2/前列腺素 H2 受体：阿霉素诱导的肾病综合征的特征和作用。
DOI：
10.1016/0925-4439(93)90104-9
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Shah,SV
中科院分区：
文献类型：
--
作者：
Mayeux,PR;Shah,SV
We have characterized the thromboxane (TX) A2/prostaglandin (PG) H2receptor in glomeruli isolated from the rat using the agonist radioligand [125I]-BPO. Binding of [125]-BOP was highly specific, stereoselective, and to a single class of high affinity binding sites (Kd= 1/16 ± 0.22 nM andBmax= 348 ± 32 fol/mg protein;n= 6). Binding of [125I]-BOP was competed for by the agonist ONO11113 (Kd= 50.8 ± 8.0 nM;n= 4) and the antagonists SQ29548 (Kd= 15.8 ± 1.0 nM;n= 3), L657925 (Kd= 12.1 ± 2.2 nM;n= 3) and L65796 (Kd= 1642 ± 135 nM;n= 3). I-BOP also produced a TXA2/PGH2receptor-mediated rise in [CA2+]iin isolated glomeruli In adriamycin-induced nephrotic syndrome in the rat, the development of proteinuria is reported to be dependent on increased renal TXA2production. We therefore examined whether or not changes in glomerular TXA2/PGH2receptors occur between control and nephrotic rats. No changes in expression of affinity of either glomerular or platelet TXA2/PGH2receptors were observed.KdandBmaxvalues for isolated isolated glomeruli were 1.45 ± 0.24 nM and 406 ± 72 fmol/gm for controls and 1.22 ± 0.25 nM and 321 ± 62 fmol/gm for nephrotic rats (n= 6).
