Quantitative PCR analysis of salivary pathogen burden in periodontitis.
Quantitative PCR analysis of salivary pathogen burden in periodontitis.
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DOI：
10.3389/fcimb.2015.00069
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发表时间：
2015
影响因子：
5.7
通讯作者：
Pussinen PJ
中科院分区：
文献类型：
--
作者：
Salminen A;Kopra KA;Hyvärinen K;Paju S;Mäntylä P;Buhlin K;Nieminen MS;Sinisalo J;Pussinen PJ
本研究旨在探讨唾液中4种主要牙周致病菌及其联合检测在牙周炎诊断中的价值。Parogene研究纳入了462名确诊为冠状动脉疾病（CAD）的齿状回受试者（平均年龄62.9 ± 9.2岁），他们接受了广泛的临床和放射学口腔检查。采用实时荧光定量PCR（qPCR）检测唾液中4种主要牙周细菌的含量。牙龈卟啉单胞菌，坦纳氏菌和中间普雷沃菌的中位唾液浓度，以及四种细菌浓度的总和，在中度至重度牙周炎的受试者中高于无至轻度牙周炎的受试者。中度牙周炎和中度至重度牙周炎受试者唾液伴放线菌聚集菌浓度中位数无显著差异。在调整了年龄、性别、糖尿病、牙齿和种植体数量的logistic回归分析中，高唾液浓度的牙龈卟啉单胞菌、T。中重度牙周炎与牙周炎的发生有显著相关性。当观察牙周炎的不同临床和放射学参数时，高浓度的牙龈卟啉单胞菌和T。牙槽骨缺损与4-5 mm牙周袋、≥6 mm牙周袋及牙槽骨缺损显著相关。高水平的T。尿道炎也与探诊出血（BOP）有关。四种细菌的组合，即，细菌负荷指数与中度至重度牙周炎相关，比值比（OR）为2.40（95%CI 1.39-4.13）。当A.排除伴放线菌后，OR提高到2.61（95% CI 1.51-4.52）。当进一步从组合中排除中间普氏菌并且仅牙龈普氏菌和T.使用的是阿西雅。牙周炎的唾液诊断具有潜力，特别是在大规模的人口研究和健康促进。累积策略似乎是有用的唾液细菌作为牙周炎的标志物的分析。
Our aim was to investigate the value of salivary concentrations of four major periodontal pathogens and their combination in diagnostics of periodontitis. The Parogene study included 462 dentate subjects (mean age 62.9 ± 9.2 years) with coronary artery disease (CAD) diagnosis who underwent an extensive clinical and radiographic oral examination. Salivary levels of four major periodontal bacteria were measured by quantitative real-time PCR (qPCR). Median salivary concentrations of Porphyromonas gingivalis, Tannerella forsythia, and Prevotella intermedia, as well as the sum of the concentrations of the four bacteria, were higher in subjects with moderate to severe periodontitis compared to subjects with no to mild periodontitis. Median salivary Aggregatibacter actinomycetemcomitans concentrations did not differ significantly between the subjects with no to mild periodontitis and subjects with moderate to severe periodontitis. In logistic regression analysis adjusted for age, gender, diabetes, and the number of teeth and implants, high salivary concentrations of P. gingivalis, T. forsythia, and P. intermedia were significantly associated with moderate to severe periodontitis. When looking at different clinical and radiographic parameters of periodontitis, high concentrations of P. gingivalis and T. forsythia were significantly associated with the number of 4–5 mm periodontal pockets, ≥6 mm pockets, and alveolar bone loss (ABL). High level of T. forsythia was associated also with bleeding on probing (BOP). The combination of the four bacteria, i.e., the bacterial burden index, was associated with moderate to severe periodontitis with an odds ratio (OR) of 2.40 (95% CI 1.39–4.13). When A. actinomycetemcomitans was excluded from the combination of the bacteria, the OR was improved to 2.61 (95% CI 1.51–4.52). The highest OR 3.59 (95% CI 1.94–6.63) was achieved when P. intermedia was further excluded from the combination and only the levels of P. gingivalis and T. forsythia were used. Salivary diagnostics of periodontitis has potential especially in large-scale population studies and health promotion. The cumulative strategy appears to be useful in the analysis of salivary bacteria as markers of periodontitis.