An antisilencer element is involved in the transcriptional regulation of the human vimentin gene
An antisilencer element is involved in the transcriptional regulation of the human vimentin gene
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DOI：
10.1016/s0378-1119(99)00046-3
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发表时间：
1999-04-01
期刊：
影响因子：
3.5
通讯作者：
Zehner, ZE
中科院分区：
文献类型：
--
作者：
Izmailova, ES;Zehner, ZE
波形蛋白是一种中间丝蛋白，通常在间充质来源的细胞中表达。先前报道人波形蛋白基因的启动子含有两个被负性区域分隔的正性作用区域(Rittling, S.R., Baserga, R., 1987. Mel. Cell. Biol. 7, 3908-3915)。在这里，详细的研究揭示了两个额外的调控元件，一个位于-717和-757之间的新的正转录元件，以及一个位于-780到-821之间的新的阻遏元件。在瞬时转染中，当与异源启动子融合时，正作用元件能够完全覆盖不同沉默元件的作用。然而，当沉默元件缺失时，该元件不会增强基因活性，因此不能被视为真正的增强子。由于它似乎克服了消音器元件的影响，因此我们将其称为反消音器元件。凝胶迁移率变化测定、UV 交联实验和西南印迹表明 105 kDa 的蛋白质与该区域特异性结合。 (C) 1999 Elsevier Science B.V. 保留所有权利。
Vimentin is an intermediate filament protein normally expressed in cells of mesenchymal origin. The promoter of the human vimentin gene was previously reported to contain two positive-acting regions, separated by a negative region (Rittling, S.R., Baserga, R., 1987. Mel. Cell. Biol. 7, 3908-3915). Here, detailed studies reveal two additional regulatory elements, a new positive transcriptional element located between -717 and -757, and a new repressor element at -780 to -821. In transient transfections, the positive-acting element is able to completely override the effect of different silencer elements when fused to a heterologous promoter. However, this element does not enhance gene activity when the silencer element is absent and thus cannot be viewed as a true enhancer. Since it appears to overcome the effect of a silencer element, we refer to it as an antisilencer element. Gel mobility shift assays, UV-cross-linking experiments, and Southwestern blots reveal that a 105-kDa protein specifically binds to this region. (C) 1999 Elsevier Science B.V. All rights reserved.