Analysis of autoantibody repertoires in sera of patients with glaucoma
Analysis of autoantibody repertoires in sera of patients with glaucoma
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DOI：
10.1177/1120672103013009-1003
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发表时间：
2003-11-01
影响因子：
1.7
通讯作者：
Pfeiffer, N
中科院分区：
文献类型：
--
作者：
Joachim, SC;Grus, FH;Pfeiffer, N
PURPOSE. Glaucoma is the second cause of blindness worldwide. It is usually considered a neurodegenerative disease. There is evidence that an autoimmune mechanism is involved in the development of glaucoma in some patients. The aim of this study was to analyze the IgG autoantibody repertoires in sera of glaucoma patients and healthy subjects.METHODS. A total of 82 patients were divided into four groups: healthy volunteers without any ocular disorders (CO, n=30), patients with primary open-angle glaucoma (POAG, n=19), ocular hypertension (OHT, n=16), and normal tension glaucoma (NTG, n=17). All groups were matched for age and gender. The sera of these patients were tested against Western blots of retinal antigens. Immunodetection was done using 4-chloro-1-naphthol staining. The autoantibody patterns were digitized and subsequently analyzed by multivariate statistical techniques.RESULTS. All patients showed different, complex staining patterns of autoantibodies against retinal antigens. There was an increase in the number of peaks in sera of patients with primary open-angle glaucoma (POAG) compared to healthy subjects (CC). Including all peaks the analysis of discriminance revealed a statistically significant difference between the patterns of POAG compared to all other groups (p
