Does stress play a role in the development of severe pancreatitis in rats?
Does stress play a role in the development of severe pancreatitis in rats?
复制标题
应激是否在大鼠重症胰腺炎的发生过程中发挥作用？
DOI：
10.1016/0016-5085(90)91107-h
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发表时间：
1990
期刊：
影响因子：
29.4
通讯作者：
H. Nawata
中科院分区：
文献类型：
--
作者：
H. Yamaguchi;T. Kimura;H. Nawata
本研究的目的是调查是否在应激中发挥作用，形态学和酶，在大鼠重症胰腺炎的发展。水浸应激5 h后，腹腔注射雨蛙肽（40 μg/kg体重），间隔1 h，诱导大鼠急性出血性胰腺炎，而单独水浸应激不引起胰腺的任何形态学和酶学变化。在该模型中，出血性胰腺炎持续发展，胰腺组织中的血清淀粉酶水平和酶原蛋白酶的活性在第一次注射雨蛙肽后5 h显著高于雨蛙肽诱导的胰腺组织。雨蛙肽对血清淀粉酶水平和酶原蛋白酶活性的影响呈剂量相关性。即使是5 μg/kg体重的雨蛙肽，在胰腺中不引起任何明显的水肿变化，也可以在水浸应激下相当好地激活胰腺组织的酶原蛋白酶，与单独40 μg/kg体重的雨蛙肽诱导的胰腺炎相比。这些结果表明，应激加速了雨蛙肽诱导的酶原蛋白酶的激活，并表明应激可能在重症胰腺炎的发生中发挥了一定作用。
The purpose of this study was to investigate whether stress plays a role, morphologically and enzymatically, in the development of severe pancreatitis in rats. Acute hemorrhagic pancreatitis was induced by two intraperitoneal injections of cerulein (40 μg/kg body wt) at intervals of 1 h under water-immersion stress for 5 h, whereas water-immersion stress alone did not induce any morphologic and enzymatic changes in the pancreas. In this model, hemorrhagic pancreatitis developed continuously, and the serum amylase level and activation of zymogen proteases in pancreatic tissue were significantly higher than in cerulein-induced pancreatic tissue 5 h after the first cerulein injection. Furthermore, the effects of cerulein on the serum amylase level and activation of zymogen proteases were dose related. Even 5 μg/kg body wt of cerulein, which did not induce any evident edematous change in the pancreas, could activate the zymogen proteases of pancreatic tissue fairly well under water-immersion stress compared with pancreatitis induced by 40 μg/kg body wt of cerulein alone. These results indicate that stress accelerates the activation of zymogen proteases induced by cerulein and suggest the possibility that stress may play some role in the development of severe pancreatitis.