PRIMARY TO QUATERNARY PROTEIN-STRUCTURE DETERMINATION WITH ELECTROSPRAY-IONIZATION AND MAGNETIC-SECTOR MASS-SPECTROMETRY
PRIMARY TO QUATERNARY PROTEIN-STRUCTURE DETERMINATION WITH ELECTROSPRAY-IONIZATION AND MAGNETIC-SECTOR MASS-SPECTROMETRY
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DOI：
10.1002/oms.1210281241
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发表时间：
1993-12-01
期刊：
影响因子：
--
通讯作者：
ANDREWS, PC
中科院分区：
文献类型：
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作者：
LOO, JA;LOO, RRO;ANDREWS, PC
利用正向几何磁扇形质谱计的电喷雾电离，研究了多电荷多肽的碰撞活化解离和非共价结合蛋白体系。高性能仪器的高分辨率能力使产品离子和分子离子物种的同位素贡献得以解析。用这种方法确定产物离子电荷态减少了从多电荷前体的产物离子质量谱解释的困难，这些前体是在大气压/真空电喷雾界面或质谱仪的碰撞腔中产生的。扩展串联质谱学实验有可能对更大的多肽进行测序。然而，观察到P物质和P物质类似物的气相产物离子异构化的证据，使产物离子光谱的解释复杂化。非共价络合物也可以用电喷雾电离磁区质谱仪进行研究。这种仪器的较高m/z范围是研究弱结合体系的主要优势，如血红素-蛋白质体系(肌红蛋白、血红蛋白)和蛋白质聚集体(刀豆蛋白A)，因为它们倾向于形成电荷状态相对较低的络合离子。
Electrospray ionization with a forward-geometry magnetic sector mass spectrometer was used for collisionally activated dissociation studies of multiply charged polypeptides and for studying non-covalently bound protein systems. The high-resolution capabilities of a high-performance instrument allow the resolution of isotopic contributions for product ions and molecular ion species. Determination of product ion charge states by this method reduces difficulties in the interpretation of product ion mass spectra from multiply charged precursors, which are generated either in the atmospheric pressure/vacuum electrospray interface or in the collision chamber of the mass spectrometer. Extended tandem mass spectrometric experiments have the potential for sequencing larger polypeptides. However, evidence for isomerization of gas-phase product ions from substance P and substance P analogues was observed, complicating the interpretation of product ion spectra. Non-covalent complexes can also be studied by electrospray ionization magnetic sector MS. The higher m/z range of such an instrument is a major advantage for studying weakly bound systems, such as heme-protein systems (myoglobin, hemoglobin) and protein aggregates (concanavalin A), because of their,tendency to form complex ions with relatively low charge states.