Combinations of two capsid regions controlling canine host range determine canine transferrin receptor binding by canine and feline parvoviruses
Combinations of two capsid regions controlling canine host range determine canine transferrin receptor binding by canine and feline parvoviruses
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DOI：
10.1128/jvi.77.18.10099-10105.2003
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发表时间：
2003-09-01
影响因子：
5.4
通讯作者：
Parrish, CR
中科院分区：
文献类型：
--
作者：
Hueffer, K;Govindasamy, L;Parrish, CR
猫泛白细胞减少症病毒（FPV）及其宿主范围变异体犬细小病毒（CPV）可与猫转铁蛋白受体（TfR）结合，而只有CPV与犬TfR结合。在FPV中引入两个CPV特异性变化（在VP2残基93和323处）赋予该病毒犬TfR结合特性并允许犬细胞感染，尽管两个变化都没有单独改变任一特性。在CPV中，VP2残基93或323与FPV序列的相互变化分别导致犬细胞感染性的适度降低。将CPV中的两个残基改变为FPV氨基酸阻断了犬细胞感染，但该病毒仍然能够以低水平结合犬TfR。这表明，两种CPV特异性变化控制犬TfR结合，但该结合并不总是足以介导感染。
Feline panleukopenia virus (FPV) and its host range variant, canine parvovirus (CPV), can bind the feline transferrin receptor (TfR), while only CPV binds to the canine TfR. Introducing two CPV-specific changes into FPV (at VP2 residues 93 and 323) endowed that virus with the canine TfR binding property and allowed canine cell infection, although neither change alone altered either property. In CPV the reciprocal changes of VP2 residue 93 or 323 to the FPV sequences individually resulted in modest reductions in infectivity for canine cells. Changing both residues in CPV to the FPV amino acids blocked the canine cell infection, but that virus was still able to bind the canine TfR at low levels. This shows that both CPV-specific changes control canine TfR binding but that binding is not always sufficient to mediate infection.