Breaking Down Lignin to High-Value Chemicals: The Conversion of Lignocellulose to Vanillin in a Gene Deletion Mutant of Rhodococcus jostii RHA1
Breaking Down Lignin to High-Value Chemicals: The Conversion of Lignocellulose to Vanillin in a Gene Deletion Mutant of Rhodococcus jostii RHA1
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DOI：
10.1021/cb400505a
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发表时间：
2013-10-01
影响因子：
4
通讯作者：
Bugg, Timothy D. H.
中科院分区：
文献类型：
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作者：
Sainsbury, Paul D.;Hardiman, Elizabeth M.;Bugg, Timothy D. H.
芳香聚合物木质素是一种可能的可再生芳香化学品来源，如果能开发出木质素分解的生物催化途径的话。Jostii红球菌RHA1是一种分解木质素的细菌，其基因组序列的获得使人们能够应用有针对性的途径工程策略来分解木质素，以生产香草醛，香草醛是一种有价值的食品/风味化学品。一株缺失香兰素脱氢酶基因的约氏根霉RHA1菌株，在含有2.5%麦草木质纤维素和0.05%葡萄糖的最低限度培养基上培养144 h，香兰素产量可达96 mg/L，同时有少量的阿魏酸和4-羟基苯甲醛。
The aromatic polymer lignin represents a possible renewable source of aromatic chemicals, if biocatalytic routes for lignin breakdown can be developed. The availability of a genome sequence for Rhodococcus jostii RHA1, a bacterium that breaks down lignin, has allowed the application of a targeted pathway engineering strategy to lignin breakdown to produce vanillin, a valuable food/flavor chemical. A gene deletion strain of R. jostii RHA1 in which the vanillin dehydrogenase gene had been deleted, when grown on minimal medium containing 2.5% wheat straw lignocellulose and 0.05% glucose, was found to accumulate vanillin with yields of up to 96 mg/L after 144 h, together with smaller amounts of ferulic acid and 4-hydroxybenzaldehyde.