UPLC‐Q‐TOF/MS‐based metabonomics study on Apostichopus Japonicus in various aquaculture models
UPLC‐Q‐TOF/MS‐based metabonomics study on Apostichopus Japonicus in various aquaculture models
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DOI：
10.1111/are.15729
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发表时间：
2021-12
影响因子：
2
通讯作者：
C. Guo;Youquan Wang;Yanglei Wu;L. Han;C. Gao;Yaqing Chang;Jun Ding
中科院分区：
文献类型：
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作者：
C. Guo;Youquan Wang;Yanglei Wu;L. Han;C. Gao;Yaqing Chang;Jun Ding
仿刺参是一种重要的经济物种，具有很高的营养价值。随着海参产业的快速发展，养殖模式也呈现多样化，导致海参品质差异明显。本研究采用超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF/MS）技术对网箱养殖、池塘养殖和底播养殖日本血吸虫体壁代谢产物进行分析。将结果与多变量分析（PLS-DA）和KEGG相结合，以确定各种水产养殖模式下的差异代谢物。结果表明，笼养日本血吸虫具有较高的脂质代谢水平，包括二十二碳五烯酸（DPA）、肾上腺素酸（Adrenic Acid）、二十二碳六烯酸（DHA）、PC（2：0/19：2）。池塘养殖的日本曲霉显示出LysoPC 16：1、LysoPC 18：3、LysoPC 20：5、异亮氨酸、3-脱羟基肉碱的高水平代谢。底播大豆表现出高水平的白烯醇、尿苷、LysoPC 22：1和肌苷代谢。KEGG途径分析表明，网箱养殖和池塘养殖日本血吸虫的代谢途径在脂类代谢、氨基酸代谢和碳水化合物代谢方面存在差异。网箱养殖和底播栽培大豆的代谢途径存在差异，主要表现在脂类代谢和核苷酸代谢方面。池塘养殖和底播栽培大豆的代谢途径存在差异，主要表现在脂类代谢和核苷酸代谢方面。本研究结果为进一步了解日本血吸虫在不同养殖模式下的代谢差异提供了新的认识。此外，这些数据可能为日本曲霉的养殖和消费提供有意义的指导。
Apostichopus japonicusis an important economic species with a high nutritional value. Aquaculture models have diversified with the rapid development of the sea cucumber industry, resulting in a distinct quality ofA.japonicus. In this study, ultra‐performance liquid chromatography–quadrupole–time‐of‐flight mass spectrometry (UPLC‐Q‐TOF/MS) technology was used to analyse body wall metabolites of cage‐cultured, pond‐cultured and bottom‐sowingA.japonicus. The results were combined with multivariate analysis (PLS‐DA) and KEGG to identify the differential metabolites under various aquaculture models. The results revealed that cage‐culturedA.japonicusshowed a high level of lipid metabolism, including Docosapentaenoic Acid (DPA), Adrenic Acid, Docosahexaenoic Acid (DHA), PC (2:0/19:2). Pond‐culturedA.japonicusshowed a high level of metabolism of LysoPC 16:1, LysoPC 18:3, LysoPC 20:5, Isoleucine, 3‐Dehydroxycarnitine. Bottom‐sowedA.japonicusshowed a high level of metabolism of Leucenol, Uridine, LysoPC 22:1 and Inosine. KEGG pathway analysis showed that the differential metabolic pathways between cage‐ and pond‐culturedA.japonicuswere in lipid metabolism, amino acid metabolism and carbohydrate metabolism. Differential metabolic pathways between cage‐cultured and bottom‐sowedA.japonicuswere in lipid metabolism and nucleotide metabolism. The differential metabolic pathways between pond‐cultured and bottom‐sowedA.japonicuswere in lipid metabolism and nucleotide metabolism. The results of this study provide new knowledge for further understanding metabolic differences ofA.japonicusin various aquaculture models. Moreover, the data may provide meaningful guidelines forA.japonicusfarming and consumption.