Large-scale degumming of ramie fibre using a newly isolated Bacillus pumilus DKS1 with high pectate lyase activity
Large-scale degumming of ramie fibre using a newly isolated Bacillus pumilus DKS1 with high pectate lyase activity
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DOI：
10.1007/s10295-008-0490-y
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发表时间：
2009-02-01
影响因子：
3.4
通讯作者：
Chakrabarti, Krishanu
中科院分区：
文献类型：
--
作者：
Basu, Snehasish;Saha, Manabendra N.;Chakrabarti, Krishanu
利用从土壤中分离到的短小芽孢杆菌菌株(DKS1)，采用酶法和化学法相结合的方法对麻皮纤维进行脱胶。分离的果胶降解菌株在低成本条件下培养24 h后，发现在小规模条件下，苎麻纤维的失重率为25%。培养上清液中果胶裂解酶活力较高，在大田条件下对400 kg麻纤维进行大规模脱胶，脱胶失重率为24%。未发现纤维素酶活性。微生物干预后的温和(0.1%)碱处理显示，苎麻纤维的失重率很高。经细菌脱胶和化学处理后的单纤维强度(CN/tex)比未脱胶(脱皮)的纤维提高了20.81%以上。扫描电子显微镜和荧光显微镜观察表明，经短小芽孢杆菌DKS1处理后的脱皮苎麻纤维表面变得非常光滑。结果表明，该工艺为脱胶麻纤维的小规模和规模化脱胶提供了一种经济、环保的方法。这项研究对纺织和造纸行业都有很大的借鉴意义。
A combined (enzymatic and chemical) process using a Bacillus pumilus strain (DKS1), isolated from the soil, was used to degum ramie bast fibres. After 24 h of incubation with the isolated pectinolytic strain using a low-cost medium, the weight loss of the ramie fibre was found to be 25% under small scale. High activity of pectate lyase was detected in the culture supernatants; 400 kg of ramie fibres was degummed with 24% weight loss in large-scale degumming under field conditions. No cellulase activity was found. Microbial intervention followed by mild (0.1%) alkali treatment showed high percentage of weight loss from the ramie fibre. Bacterial degumming followed by chemical treatment resulted in an increase of single fibre tenacity (cN/tex) by more than 20.81% as compared to non-degummed (decorticated) fibre samples. Scanning electron micrographs (SEM) and fluorescence microscope showed that after Bacillus pumilus DKS1 treatment the surface of the decorticated ramie fibre becomes very smooth. These results indicate the process provides an economical and eco-friendly method for the small scale as well as large-scale degumming of decorticated ramie fibre. This study has great relevance to the textile as well as paper industry.