SnRK1 (SNF1-related kinase 1) has a central role in sugar and ABA signalling in Arabidopsis thaliana
SnRK1 (SNF1-related kinase 1) has a central role in sugar and ABA signalling in Arabidopsis thaliana
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DOI：
10.1111/j.1365-313x.2009.03871.x
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发表时间：
2009-07-01
期刊：
影响因子：
7.2
通讯作者：
Thomas, Martine
中科院分区：
文献类型：
--
作者：
Jossier, Mathieu;Bouly, Jean-Pierre;Thomas, Martine
蛋白激酶SNF 1/AMPK/SnRK 1是丝氨酸/苏氨酸激酶的一个亚家族，作为代谢物传感器，不断适应代谢对能量的供应和需求。在酿酒酵母中，SNF 1复合物是葡萄糖饥饿调节反应的核心成分。AMP活化蛋白激酶（AMPK）是SNF 1的哺乳动物同源物，在细胞和全身水平的能量稳态调节中起核心作用。在拟南芥中，SnRK1.1和SnRK1.2最近被描述为压力和能量信号传导的转录网络的中心整合子。在这项研究中，生化分析建立了SnRK1.1作为主要的SnRK 1亚型在分离的细胞和叶。为了阐明SnRK1.1在拟南芥中的功能，获得了过量表达SnRK1.1的转基因植物。对这些植物的遗传、生化、生理和分子分析表明，SnRK1.1与糖和阿坝信号通路有关。在35 S：SnRK1.1转基因株系中观察到淀粉和可溶性糖含量的改变。我们的研究还揭示了硝酸还原酶或ADP-葡萄糖焦磷酸化酶等必需酶的活性以及几种糖调节基因的表达的改变，证实了蛋白激酶SnRK 1在代谢调节中的核心作用。
P>The proteins kinases SNF1/AMPK/SnRK1 are a subfamily of serine/threonine kinases that act as metabolite sensors to constantly adapt metabolism to the supply of, and demand for, energy. In the yeast Saccharomyces cerevisiae, the SNF1 complex is a central component of the regulatory response to glucose starvation. AMP activated protein kinase (AMPK) the mammalian homologue of SNF1, plays a central role in the regulation of energy homeostasis at the cellular as well as the whole-body levels. In Arabidopsis thaliana, SnRK1.1 and SnRK1.2 have recently been described as central integrators of a transcription network for stress and energy signalling. In this study, biochemical analysis established SnRK1.1 as the major SnRK1 isoform both in isolated cells and leaves. In order to elucidate the function of SnRK1.1 in Arabidopsis thaliana, transgenic plants over-expressing SnRK1.1 were produced. Genetic, biochemical, physiological and molecular analyses of these plants revealed that SnRK1.1 is implicated in sugar and ABA signalling pathways. Modifications of the starch and soluble sugar content were observed in the 35S:SnRK1.1 transgenic lines. Our studies also revealed modifications of the activity of essential enzymes such as nitrate reductase or ADP-glucose pyrophosphorylase, and of the expression of several sugar-regulated genes, confirming the central role of the protein kinase SnRK1 in the regulation of metabolism.