Trait-Based Comparison of Coral and Sponge Microbiomes
Trait-Based Comparison of Coral and Sponge Microbiomes
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DOI：
10.1038/s41598-020-59320-9
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发表时间：
2020-02-11
影响因子：
4.6
通讯作者：
Lesser, Michael P.
中科院分区：
文献类型：
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作者：
Fiore, Cara L.;Jarett, Jessica K.;Lesser, Michael P.
珊瑚和海绵含有多种微生物群落，它们是宿主功能不可或缺的一部分。虽然珊瑚和海绵的微生物组的分类多样性已经得到了很好的确立，但它们的功能作用却不太清楚。目前尚不清楚无脊椎动物宿主中共生的相似性是否会导致功能相似的微生物组，或者每个宿主内宿主发育和环境驱动的微生境的差异是否会形成功能不同的群落。在这里，我们解决了这个问题，使用元转录组学和16S rRNA基因分析技术，比较两个不同门的宿主生物的微生物组。我们的研究结果表明，碳，氮，硫同化，有氧氮循环功能相似。此外，两种宿主之间的途径覆盖率或丰度几乎没有统计学差异。例如，我们观察到较高的覆盖率的膦酸盐和铁载体代谢途径的星星珊瑚，Montastraea cavernosa，而有较高的覆盖率的氯代烷烃代谢的巨型桶海绵，Xestospongia穆塔。与碳固定途径相关的基因在M. cavernosa，而X.穆塔组脂肪酸代谢途径丰度较高。基于16S rRNA基因谱分析的宏基因组预测是相似的，并且两种宿主的宏转录组和宏基因组预测之间具有高度相关性。我们的研究结果强调了几种代谢途径，尽管这两种微生物之间存在分类学差异，但这些代谢途径在珊瑚和海绵微生物中表现出功能相似性，以及每个宿主中一些基于微生物的代谢的潜在专业化。
Corals and sponges harbor diverse microbial communities that are integral to the functioning of the host. While the taxonomic diversity of their microbiomes has been well-established for corals and sponges, their functional roles are less well-understood. It is unclear if the similarities of symbiosis in an invertebrate host would result in functionally similar microbiomes, or if differences in host phylogeny and environmentally driven microhabitats within each host would shape functionally distinct communities. Here we addressed this question, using metatranscriptomic and 16S rRNA gene profiling techniques to compare the microbiomes of two host organisms from different phyla. Our results indicate functional similarity in carbon, nitrogen, and sulfur assimilation, and aerobic nitrogen cycling. Additionally, there were few statistical differences in pathway coverage or abundance between the two hosts. For example, we observed higher coverage of phosphonate and siderophore metabolic pathways in the star coral, Montastraea cavernosa, while there was higher coverage of chloroalkane metabolism in the giant barrel sponge, Xestospongia muta. Higher abundance of genes associated with carbon fixation pathways was also observed in M. cavernosa, while in X. muta there was higher abundance of fatty acid metabolic pathways. Metagenomic predictions based on 16S rRNA gene profiling analysis were similar, and there was high correlation between the metatranscriptome and metagenome predictions for both hosts. Our results highlight several metabolic pathways that exhibit functional similarity in these coral and sponge microbiomes despite the taxonomic differences between the two microbiomes, as well as potential specialization of some microbially based metabolism within each host.