Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection
Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection
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DOI：
10.1186/1743-422x-7-361
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发表时间：
2010-12-06
期刊：
影响因子：
4.8
通讯作者：
Parra, Beatriz
中科院分区：
文献类型：
--
作者：
Osorio, Lyda;Ramirez, Meleny;Parra, Beatriz
背景资料：我们比较了市售的NS 1为基础的登革热测试的诊断准确性和再现性，并探讨了影响其sensitivity.Methods的因素：配对分析310个样本先前表征为阳性（n = 218）和阴性（n = 92）的病毒分离和/或RT-PCR和/或IgM血清转化。由两名观察员用Platelia（TM）Dengue NS 1 Ag、第二代Pan-E（TM）Dengue Early ELISA、SD Dengue NS 1 Ag ELISA、Dengue NS 1 Ag STRIP（TM）和SD BIOLINE（TM）Dengue Duo测试掩蔽的样品（NS1/IgM/IgG）。SD BIOLINE（TM）NS 1/IgM/IgG的灵敏度最高（80.7% 95% CI 75-85.7），似然比为7.4（95% CI 4.1-13.8）和0.21（95% CI 0.16-0.28）。ELISA格式测试显示出相当的灵敏度;均低于75%。STRIP（TM）和SD NS 1的灵敏度甚至更低（
Background: We compared the diagnostic accuracy and reproducibility of commercially available NS1-based dengue tests and explored factors influencing their sensitivities.Methods: Paired analysis of 310 samples previously characterized as positive (n = 218) and negative (n = 92) for viral isolation and/or RT-PCR and/or IgM seroconversion. Masked samples were tested by two observers with Platelia (TM) Dengue NS1 Ag, second generation Pan-E (TM) Dengue Early ELISA, SD Dengue NS1 Ag ELISA, Dengue NS1 Ag STRIP (TM), and SD BIOLINE (TM) Dengue Duo (NS1/IgM/IgG).Results: SD BIOLINE (TM) NS1/IgM/IgG had the highest sensitivity (80.7% 95% CI 75-85.7) with likelihood ratios of 7.4 (95% CI 4.1-13.8) and 0.21 (95% CI 0.16-0.28). The ELISA-format tests showed comparable sensitivities; all below 75%. STRIP (TM) and SD NS1 had even lower sensitivities (