Differential inhibition of T and B cell responses to individual antigenic determinants in orally tolerized mice.
Differential inhibition of T and B cell responses to individual antigenic determinants in orally tolerized mice.
复制标题
口服耐受小鼠中 T 细胞和 B 细胞对个体抗原决定簇反应的差异抑制。
DOI：
--
复制
发表时间：
1994
影响因子：
4.4
通讯作者：
S. Kaminogawa
中科院分区：
文献类型：
--
作者：
S. Hachimura;Y. Fujikawa;A. Enomoto;S. M. Kim;A. Ametani;S. Kaminogawa
通过口服外源性抗原可以诱导全身免疫的免疫耐受，称为口服耐受。我们研究了在口服耐受性中个体抗原决定簇之间的耐受程度是否存在差异。牛α s1-酪蛋白是牛奶中的一种主要蛋白质，作为饮食的组成部分喂养可诱导小鼠的口服耐受。然而，通过随后用抗原免疫，可以在喂食α s1酪蛋白的动物中引发弱抗体反应。我们研究了这种抗α-s1-酪蛋白抗体在α-s1-酪蛋白喂养的小鼠中产生的精细特异性。结果表明，不同的B细胞决定簇对抗体应答的抑制作用存在差异。还可以观察到T细胞应答的差异抑制。显性决定簇特异性T细胞优先耐受，而隐性决定簇特异性T细胞逃避口服耐受。我们的研究结果意味着抗原呈递的重要性，这种差异抑制抗原决定簇。我们认为，口服给药的抗原不会诱导耐受的B和T淋巴细胞库，可能会引起有害的食物过敏。
Immunological tolerance of systemic immunity can be induced by the oral administration of an exogenous antigen, which is termed oral tolerance. We examined whether there was a difference in the degree of tolerance between individual antigenic determinants in oral tolerance. Feeding bovine alpha s1-casein, a major protein in cow's milk, as a constituent of the diet induces oral tolerance in mice. However, a weak antibody response can be elicited in the alpha s1-casein-fed animals by subsequent immunization with the antigen. We examined the fine specificity of such anti-alpha s1-casein antibodies produced in alpha s1-casein-fed mice. The results demonstrated that there was a difference in the inhibition of antibody response between different B cell determinants. Differential inhibition could also be observed for T cell responses. T cells specific for dominant determinants were preferentially tolerized, while those for cryptic determinants escaped oral tolerance. Our results imply the importance of antigen presentation for this differential inhibition of antigenic determinants. We suggest that orally administered antigen does not induce tolerance to some of the B and T lymphocyte repertoire which could potentially induce harmful food hypersensitivity.