Stable isotopic analysis of porcine, bovine, and ovine heparins.
Stable isotopic analysis of porcine, bovine, and ovine heparins.
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猪，牛和卵巢肝素的稳定同位素分析。
DOI：
10.1002/jps.24134
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发表时间：
2015-02
影响因子：
3.8
通讯作者：
Linhardt, Robert J.
中科院分区：
文献类型：
--
作者：
Jasper, John P.;Zhang, Fuming;Poe, Russell B.;Linhardt, Robert J.
关键词：
肝素来源的评估正成为制药行业及其监管机构的主要关注点。对5种不同动物源性肝素进行批次特异性[碳（δ 13 C）、氮（δ 15 N）、氧（δ 18 O）、硫（δ 34 S）和氢（δD）]稳定同位素组成分析。测量很容易让他们分化成组和/或亚组的基础上，他们的同位素来源。主成分分析表明，δ 13 C和δ 18 O的双变量图是最好的单，双变量图，导致最大的区分能力时，只有两个稳定的同位素来描述数据集中的变化。稳定同位素分析表明，（i）这些高度硫酸化多糖的稳定同位素测量（MW ~15 kDa）天然产物（ii）在双变量图中，δ 13 C对δ 18 O图揭示了美国饲养的猪与欧洲和中国饲养的猪的来源分化的明确关系;（iii）δD-δ 18 O图揭示了基于水的水文-环境同位素的源分化的最明确的关系（D/H和18 O/16 O），以及（iv）δ 15 N与δ 18 O和δ 34 S与δ 18 O的关系都非常相似，可能反映了不同肝素生产商使用的食物来源。
The assessment of provenance of heparin is becoming a major concern for the pharmaceutical industry and its regulatory bodies. Batch-specific [carbon (δ13C), nitrogen (δ15N), oxygen (δ18O), sulfur (δ34S), and hydrogen (δD)] stable-isotopic compositions of five different animal-derived heparins were performed. Measurements readily allowed their differentiation into groups and/or subgroups based on their isotopic provenance. Principle component analysis showed that a bivariate plot of δ13C and δ18O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set. Stable-isotopic analyses revealed that (i) stable-isotope measurements on these highly-sulfated polysaccharide (MW ~15 kDa) natural products (“biologics”) were feasible; (ii) in bivariate plots, the δ13C versus δ18O plot reveals a well-defined relationship for source differentiation of hogs raised in the US from hogs raised in Europe and China; (iii) the δD versus δ18O plot revealed the most well-defined relationship for source differentiation based on the hydrologic-environmental isotopes of water (D/H and 18O/16O), and (iv) the δ15N versus δ18O and δ34S versus δ18O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
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DOI：
10.1016/1053-0770(91)90118-d
发表时间：
1991-10-01
影响因子：
2.8
作者：
Boldt, J;Zickmann, B;Hempelmann, G
通讯作者：
Hempelmann, G
影响因子：
4.3
作者：
Concannon, Sean P.;Wimberley, P. Brett;Workman, Wesley E.
通讯作者：
Workman, Wesley E.
影响因子：
5
作者：
Bhaskar, Ujjwal;Sterner, Eric;Hickey, Anne Marie;Onishi, Akihiro;Zhang, Fuming;Dordick, Jonathan S.;Linhardt, Robert J.
通讯作者：
Linhardt, Robert J.
DOI：
10.1177/106002808602000509
发表时间：
1986-05-01
期刊：
DRUG INTELLIGENCE & CLINICAL PHARMACY
影响因子：
--
作者：
BAILEY, RT;URSICK, JA;REICH, JW
通讯作者：
REICH, JW
影响因子：
2.9
作者：
Houiste, Celine;Auguste, Cecile;Anger, Pascal
通讯作者：
Anger, Pascal