Immunolocalization of proteoglycans and bone-related noncollagenous glycoproteins in developing acellular cementum of rat molars
Immunolocalization of proteoglycans and bone-related noncollagenous glycoproteins in developing acellular cementum of rat molars
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DOI：
10.1007/s00441-004-0896-4
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发表时间：
2004-09-01
影响因子：
3.6
通讯作者：
Wakita, M
中科院分区：
文献类型：
--
作者：
Yamamoto, T;Domon, T;Wakita, M
为了阐明蛋白聚糖（pg）、骨涎蛋白（BSP）和骨桥蛋白（OPN）在骨水泥发生中的作用，采用免疫过氧化物酶法研究了它们在大鼠磨牙发育和建立的脱细胞骨水泥中的分布。为了表征PGs，使用了针对5种糖胺聚糖（GAGs）、4-硫酸软骨素（C4S）、6-硫酸软骨素（C6S）、无硫酸软骨素（C0S）、硫酸皮聚糖（DS）和硫酸角蛋白（KS）的抗体。同时进行常规组织学染色。随着牙本质矿化的开始，牙本质表面出现了一层苏木精染色的缺原纤维层。随后，原始的主纤维附着在最初的骨质上。当含有外源性纤维的脱细胞牙骨质覆盖初始牙骨质时，初始牙骨质形成牙骨质-牙本质交界处。在骨水泥形成早期进行免疫组化，初始骨水泥对C4S、C6S、C0S、BSP和OPN具有强烈的免疫反应。在初始牙骨质嵌入后，牙骨质-牙本质交界处和牙骨质对任何GAG物种都没有免疫反应。然而，牙骨质和牙骨质-牙本质交界处对BSP的免疫反应一致。尽管牙骨质-牙本质交界处对OPN的免疫反应一致，但剩余的牙骨质没有明显的免疫反应。因此，初始的脱细胞骨质形成需要pg、BSP和OPN的密集积累，这可能与矿化过程独立于胶原原纤维和初始主纤维附着有关。
To elucidate the roles of proteoglycans (PGs), bone sialoprotein (BSP), and osteopontin (OPN) in cementogenesis, their distribution was investigated in developing and established acellular cementum of rat molars by an immunoperoxidase method. To characterize PGs, antibodies against five species of glycosaminoglycans (GAGs), chondroitin-4-sulfate (C4S), chondroitin-6-sulfate (C6S), unsulfated chondroitin (C0S), dermatan sulfate (DS), and keratan sulfate (KS) were used. Routine histological staining was also applied. With onset of dentin mineralization, the initial cementum appeared on the dentin surface as a hematoxylin-stained fibril-poor layer. Subsequently, primitive principal fibers attached to the initial cementum. As the acellular cementum containing extrinsic fibers covered the initial cementum, the initial cementum formed the cemento-dentinal junction. Following immunohistochemistry at the earliest time of cementogenesis, the initial cementum was intensely immunoreactive for C4S, C6S, C0S, BSP, and OPN. After the initial cementum was embedded, neither the cemento-dentinal junction nor the cementum was immunoreactive for any GAG species. However, the cementum and cemento-dentinal junction were consistently immunoreactive for BSP. Although the cemento-dentinal junction was consistently immunoreactive for OPN, the remaining cementum showed no significant immunoreactivity. Thus, initial acellular cementogenesis requires a dense accumulation of PGs, BSP, and OPN, which may be associated with the mineralization process independently of collagen fibrils and initial principal fiber attachment.