Time course changes of vasopressin-induced enlargement of the rat intrastrial space and the effects of a vasopressin type 2 antagonist
Time course changes of vasopressin-induced enlargement of the rat intrastrial space and the effects of a vasopressin type 2 antagonist
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DOI：
10.1080/00016480802419115
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发表时间：
2009-01
影响因子：
1.4
通讯作者：
M. Nishimura;A. Kakigi;T. Takeda;T. Okada;K. Doi
中科院分区：
文献类型：
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作者：
M. Nishimura;A. Kakigi;T. Takeda;T. Okada;K. Doi
结论：加压素注射后20 min，脊髓内间隙明显扩大，在注射前给予OPC-31260可减轻脊髓内间隙的扩大。这些结果表明，VP通过水通道蛋白（AQP）2增加了水从外淋巴液到基底细胞的内流，并导致内淋巴积水的形成。目的：研究VP注射后血管纹变化的时间过程以及OPC-31260对VP注射引起的实验诱导的脑内空间扩大的影响。材料和方法：在时间过程研究中，Wistar大鼠皮下注射VP 50 μg/kg。在VP注射后10、20、30和60 min收获血管纹标本。对于OPC-31260给药，动物在皮下注射50 µg/kg VP前1 h经口给予100 mg/kg OPC-31260。VP注射后20 min收集标本。用透射电子显微镜观察这些标本。结果：在时间过程研究中，10、20、30和60 min时，椎间隙扩大的发生率分别为50%、100%、25%和0%。在OPC-31260给药研究中，血管纹未显示形态学变化。
Conclusion: The intrastrial space was enlarged remarkably at 20 min after vasopressin (VP) injection, and this enlargement of the intrastrial space was reduced by administration of OPC-31260 before VP injection. These results suggest that VP increases the influx of water from the perlymph to the basal cells via aquaporin (AQP) 2 and causes the formation of endolymphatic hydrops. Objectives: To investigate a time course of changes of the stria vascularis after VP injection and the influence of OPC-31260 on experimentally induced enlargement of the intrastrial space caused by VP injection. Materials and methods: In the time course study, Wistar rats were injected with 50 µg/kg of VP subcutaneously. The stria vascularis specimens were harvested at 10, 20, 30, and 60 min after VP injection. For OPC-31260 administration, animals were administered 100 mg/kg of OPC-31260 orally 1 h before receiving 50 µg/kg of VP subcutaneously. The specimens were harvested 20 min after VP injection. These specimens were observed using transmission electron microscopy. Results: In the time course study, the incidence of intrastrial space enlargement was 50%, 100%, 25%, and 0% for 10, 20, 30, and 60 min, respectively. In the study with OPC-31260 administration, the stria vascularis showed no morphological changes.