PERMSELECTIVITY OF GLOMERULAR CAPILLARY WALL - STUDIES OF EXPERIMENTAL GLOMERULONEPHRITIS IN RAT USING DEXTRAN SULFATE
PERMSELECTIVITY OF GLOMERULAR CAPILLARY WALL - STUDIES OF EXPERIMENTAL GLOMERULONEPHRITIS IN RAT USING DEXTRAN SULFATE
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DOI：
10.1172/jci108396
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发表时间：
1976-01-01
影响因子：
15.9
通讯作者：
BRENNER, BM
中科院分区：
文献类型：
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作者：
BENNETT, CM;GLASSOCK, RJ;BRENNER, BM
为了确定肾小球损伤后血清蛋白过滤增加是否是肾小球毛细血管壁静电性质改变的结果，在9只Munich-Wistar大鼠肾毒性血清肾炎（NSN）的早期自体阶段测定了阴离子聚合物硫酸葡聚糖的清除率。尽管有肾小球损伤的组织学证据，尽管肾小球毛细血管超滤系数显著降低至正常值的约1/3，但NSN大鼠的全肾和单肾单位肾小球滤过率正常。在相同的分子半径范围内，硫酸葡聚糖的平均清除率分数显著大于先前在正常Munich-Wistar大鼠中发现的值。具有与血清白蛋白相同分子半径的硫酸葡聚糖分子的清除分数（. 36.安从0.015 ± 0.015显著增加。0.005（SEM），非肾炎对照组为0.24 ±。0.003（SEM）（P < 0.001）。与正常对照组相比，NSN大鼠肾小球的唾液蛋白含量（通过胶体铁反应估计）降低。在肾小球病中观察到的阴离子血清蛋白（如白蛋白）的异常过滤至少部分是肾小球毛细血管壁固定负电荷丢失的结果。
To determine whether the increased filtration of serum proteins after glomerular injury is the consequence of altered electrostatic properties of the glomerular capillary wall, fractional clearances of the anionic polymer, dextran sulfate, were measured in 9 Munich-Wistar rats in the early autologous phase of nephrotoxic serum nephritis (NSN). Whole kidney and single nephron glomerular filtration rates were normal in NSN rats despite histological evidence of glomerular injury, and despite a marked reduction in the glomerular capillary ultrafiltration coefficient to approximately 1/3 of normal. The mean fractional clearances for dextran sulfate over the same range of molecular radii were significantly greater than those found previously for normal Munich-Wistar rats. The fractional clearance of dextran sulfate molecules of the same molecular radius as serum albumin (.apprx. 36 .ANG.) was increased markedly, from 0.015 .+-. 0.005 (SEM) in non-nephritic controls to 0.24 .+-. 0.003 (SEM) in NSN (P < 0.001). The sialoprotein content of glomeruli, estimated by the colloidal Fe reaction, was reduced in NSN rats as compared to normal controls. The abnormal filtration of anionic serum proteins, such as albumin, seen in glomerulopathies is, at least in part, the consequence of loss of fixed negative charges from the glomerular capillary wall.