AMNIOTIC BAND SYNDROME IN FETAL LAMBS .1. FETOSCOPIC RELEASE AND MORPHOMETRIC OUTCOME
AMNIOTIC BAND SYNDROME IN FETAL LAMBS .1. FETOSCOPIC RELEASE AND MORPHOMETRIC OUTCOME
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DOI：
10.1016/0022-3468(95)90324-0
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发表时间：
1995-07-01
影响因子：
2.4
通讯作者：
CONNELLY, RJ
中科院分区：
文献类型：
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作者：
CROMBLEHOLME, TM;DIRKES, K;CONNELLY, RJ
建立了羊膜带综合征（ABS）的胎羊模型，以研究带状四肢的病理生理学，并评估子宫内治疗的可能性和功能恢复的可能性。8只胎羊在妊娠100天时用脐带带捆绑四肢。两个羔羊流产后，开放式胎儿手术。两个未手术的新生羔羊的肢体作为对照，除了五个假手术的对照肢体，没有应用带。9条肢体被捆绑而不减少通过激光多普勒评估的血流量（组1），6条肢体被类似地捆绑并在妊娠125天时通过胎儿镜释放。四肢被捆绑，通过激光多普勒流量计测量，肢体血流平均减少18.7%（第2组）。出生后不久，将羔羊处死，并对节段性肢体长度、周长、关节活动范围和组织学进行评价。出生时，带状肢体表现出明显的肌肉发达水肿和带状远端无毛。节段性肢体测量显示，带状肢体远端前肢短于对照组（10.97 ± 0.59 vs 12.98 ± 0.69，P
A fetal lamb model of amniotic band syndrome (ABS) was developed to study the pathophysiology of banded extremities and evaluate the possibility of in utero treatment with the potential for functional recovery. Eight fetal lambs underwent banding of their extremities with umbilical tape at 100 days' gestation. Two lambs aborted after the open fetal surgery. The limbs of two unoperated newborn lambs served as controls in addition to five sham-operated control limbs that had no bands applied. Nine limbs were banded without reducing blood flow assessed by laser doppler (group 1), and 6 limbs were similarly banded and released fetoscopically at 125 days' gestation. Four limbs were banded, with a mean reduction in blood flow to the limb of 18.7% (group 2) by laser doppler flowmeter. Shortly after birth the lambs were killed, and segmental limb length, circumference, joint range of motion, and histology were evaluated. At birth, banded limbs showed marked brawny edema and absence of wool distal to the band, Segmental limb measurements showed shorter distal forelimbs in banded limbs compared with controls (10.97 +/- 0.59 versus 12.98 +/- 0.69, P