Static time‐of‐flight secondary ion mass spectrometry and x‐ray photoelectron spectroscopy characterization of adsorbed albumin and fibronectin films
Static time‐of‐flight secondary ion mass spectrometry and x‐ray photoelectron spectroscopy characterization of adsorbed albumin and fibronectin films
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DOI：
10.1002/sia.1101
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发表时间：
2001-08
影响因子：
1.7
通讯作者：
C. Tidwell;D. Castner;S. Golledge;B. Ratner;Klaus Meyer;B. Hagenhoff;A. Benninghoven
中科院分区：
文献类型：
--
作者：
C. Tidwell;D. Castner;S. Golledge;B. Ratner;Klaus Meyer;B. Hagenhoff;A. Benninghoven
静态飞行时间二次离子质谱 (ToF-SIMS)、单色 X 射线光电子能谱 (XPS) 和 125I 放射性标记已用于表征吸附在钛、金、聚四氟乙烯和射频上的白蛋白膜。辉光放电沉积四氟乙烯（TFE）基板。还对白蛋白和纤连蛋白膜进行了比较。静态ToF-SIMS实验中检测到的特征氨基酸质量片段（铵离子）的强度取决于蛋白质类型、底物类型和吸附条件，证明了静态ToF-SIMS探测吸附蛋白质膜结构的灵敏度。基于白蛋白和纤连蛋白的结果，静态ToF-SIMS可以提供有关吸附蛋白质的身份及其构象、方向、变性等的信息。X射线光电子能谱可以区分纯蛋白质薄膜，但静态ToF-SIMS较高的分子特异性比XPS更适合检查复杂的蛋白质薄膜。 125I放射性标记实验和XPS氮原子百分比用于定量吸附蛋白质的量。版权所有 © 2001 约翰·威利父子有限公司
Static time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS), monochromatized x‐ray photoelectron spectroscopy (XPS) and 125I radiolabeling have been used to characterize albumin films adsorbed onto titanium, gold, polytetrafluoroethylene and r.f. glow discharge‐deposited tetrafluoroethylene (TFE) substrates. A comparison between albumin and fibronectin films also was made. The intensities of characteristic amino acid mass fragments (immonium ions) detected in the static ToF‐SIMS experiments depended on the protein type, the substrate type and the adsorption conditions, demonstrating the sensitivity of static ToF‐SIMS for probing the structure of adsorbed protein films. Based on the results from albumin and fibronectin, static ToF‐SIMS can provide information about the identity of adsorbed proteins and their conformation, orientation, denaturation, etc. X‐ray photoelectron spectroscopy can distinguish pure protein films, but the higher molecular specificity of static ToF‐SIMS is more useful than XPS for examining complex protein films. The 125I radiolabeling experiments and the XPS atomic percentage of nitrogen were used to quantify the amount of adsorbed protein. Copyright © 2001 John Wiley & Sons, Ltd.