Cowdria ruminantium antibodies in acaricide-treated and untreated cattle exposed to Amblyomma variegatum ticks in The Gambia
Cowdria ruminantium antibodies in acaricide-treated and untreated cattle exposed to Amblyomma variegatum ticks in The Gambia
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DOI：
10.1023/a:1010645927535
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发表时间：
2000-01-01
影响因子：
2.2
通讯作者：
Jongejan, F
中科院分区：
文献类型：
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作者：
Mattioli, RC;Bah, M;Jongejan, F
以反义考氏杆菌主要抗原蛋白1片段B(MAP1-B)为基础，建立了间接酶联免疫吸附试验(EL ISA)，用于评估冈比亚牛的血清阳性率。对20头N‘Dama牛和20头Gobra Zebu牛进行了为期12个月的氟氰菊酯处理和10个月的无杀螨剂处理的监测。20头N‘Dama牛和20头戈布拉牛作为未经处理的对照组。在驱螨期内，N‘Dama牛和Gobra牛的累计阳性血清比例分别为25.6+/-5.6%(+/-可信区间)和34.7+/-6.8%，未处理牛的阳性血清比例分别为52.2+/-6.9%和61.4+/-7.3%。在品种内，处理牛和未处理牛的抗体患病率差异显著(P<；0.001)，但品种间差异不显著。在停止使用杀螨剂后的10个月内，以前处理过的N‘Dama和Gobra牛的血清阳性样本比例有所增加。在处理过的和未处理过的动物中，阳性血清反应的高峰都出现在花纹夜蛾成虫活动期期间和之后。处理过的N‘Dama和Gobra牛的累积血清阳性率分别为32.6+/-6.9%和44.7+/-8.5%，未处理的N’Dama牛和Gobra牛的累积血清阳性率分别为38.6+/-7.2%和65.3+/-8.4%。在整个研究期间，在N‘Dama品种内，以前处理过的牛的血清阳性率与未处理过的牛没有不同。相反，在戈布拉品种中，未经处理的动物的阳性血清反应数量高于先前处理的牛(P<；0.002)。如有必要，这些结果为在冈比亚设计与牛品种有关的变色黄牛和心水控制策略提供了支持。
An indirect enzyme-linked immunosorbent assay (ELISA), based on the major antigenic protein 1 fragment B (MAP1-B) of Cowdria ruminantium, was used to assess seroprevalence in cattle in The Gambia. Two groups of 20 N'Dama and 20 Gobra zebu cattle were monitored for 12 months with flumethrin treatment and for another 10 months without acaricidal treatment. Two groups of 20 N'Dama and 20 Gobra cattle served as untreated controls. During the period of acaricidal treatment, the cumulative proportions of positive serum samples were 25.6 +/- 5.6% ( +/- confidence interval) and 34.7 +/- 6.8% in treated N'Dama and Gobra cattle respectively; the proportion of positive sera in untreated cattle was 52.2 +/- 6.9% in N'Damas and 61.4 +/- 7.3% in Gobras. Within breed, difference in antibody prevalence between treated and untreated cattle was significant (P < 0.001) but between breed differences were not significant. In the 10 months following suspension of acaricide application, there was an increase of proportion of positive serum samples in previously treated N'Dama and Gobra cattle. In both previously treated and untreated animals the peak of positive seroreactions occurred during and subsequent to the period of activity of Amblyomma variegatum adults. Cumulative seroprevalences in previously treated N'Dama and Gobra cattle were 32.6 +/- 6.9% and 44.7 +/- 8.5%, respectively; in untreated animals seroprevalence was 38.6 +/- 7.2% in N'Dama and 65.3 +/- 8.4% in Gobra cattle. Throughout the study period, within the N'Dama breed, the seropositive rate in previously treated cattle did not differ from that in untreated animals. Conversely, within the Gobra breed, the number of positive seroreactions was higher (P < 0.002) in untreated animals than in previously treated cattle. These results provide a support for designing A. variegatum and heartwater control strategies, if necessary, in The Gambia in relation to cattle breeds.