Identification of high-affinity binding sites for the hepta-β-glucoside elicitor in membranes of the model legumes Medicago truncatula and Lotus japonicus
Identification of high-affinity binding sites for the hepta-β-glucoside elicitor in membranes of the model legumes Medicago truncatula and Lotus japonicus
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DOI：
10.1007/s004250000325
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发表时间：
2000-09-01
期刊：
影响因子：
4.3
通讯作者：
Hahn, MG
中科院分区：
文献类型：
--
作者：
Côté, F;Roberts, KA;Hahn, MG
先前的研究已经导致大豆中七-β-葡萄糖苷诱导子的特异性、高亲和力结合位点的鉴定和表征。对植物物种的激发子结合活性的调查显示，在测试的植物中，七-β-葡糖苷激发子仅被属于豆科的植物识别。我们详细描述了模式豆科植物蒺藜苜蓿中的葡聚糖激发子结合位点，并部分地描述了该场地的特徴。这些位点具有与大豆中的位点非常相似的特征，解离常数分别为4.7和8.9 nM。从两种植物的激发子结合位点是稳定的溶解过程中与非离子烷基糖苷洗涤剂。然而.在结合位点的丰度和它们对七-β-葡糖苷诱导子的结构相关类似物的选择性方面观察到差异。我们的研究结果表明，类似的，但也许不完全相同。七-β-葡糖苷诱导子的结合位点存在于多种豆科植物中，但不存在于豆科以外的植物中。
Previous studies have led to the identification and characterization of specific, high-affinity binding sites for a hepta-beta-glucoside elicitor in soybean. A survey of plant species for elicitor-binding activity reveals that among the plants tested, the hepta-beta-glucoside elicitor is only recognized by plants belonging to the legume family. We have characterized in detail the glucan elicitor-binding site in the model legume Medicago truncatula Gaertn., and partially characterized the site in torus japonicus. These sites have characteristics that ale very similar to the one in soybean, with dissociation constants of 4.7 and 8.9 nM respectively. The elicitor-binding sites from both plants are stable during solubilization with non-ionic alkylglycoside detergents. However. differences are observed in the abundance of the binding sites and their selectivity towards structurally related analogues of the hepta-beta-glucoside elicitor. Our results suggest that similar, but perhaps not identical. binding sites for the hepta-beta-glucoside elicitor exist, in diverse legumes, but not in plants outside of the legume family.