The influence of PSA-RNA yield on the analysis of expressed prostatic secretions (EPS) for prostate cancer diagnosis.
The influence of PSA-RNA yield on the analysis of expressed prostatic secretions (EPS) for prostate cancer diagnosis.
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发表时间：
2013-02
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通讯作者：
C. Whelan;L. Crocitto;M. Kawachi;Kevin G. Chan;David D. Smith;T. Wilson;Steven M. Smith
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作者：
C. Whelan;L. Crocitto;M. Kawachi;Kevin G. Chan;David D. Smith;T. Wilson;Steven M. Smith
前列腺癌患者的前列腺腔特异性抗原（PSA）由于基底膜和腺上皮受损而进入循环。鉴于前列腺按摩过程中前列腺细胞的过度动员可能影响诊断测试的正常化，我们研究了前列腺癌活检患者的前列腺分泌物（EPS）中的PSA mRNA水平，以确定前列腺按摩过程中前列腺细胞是否优先从前列腺癌患者中动员。材料与方法采用定量逆转录聚合酶链反应（qRT-PCR）检测前列腺癌患者EPS中GAPDH、PSA、TMPRSS 2：ERG和PCA 3的RNA水平。结果前列腺癌患者EPS中PSA mRNA水平显著升高。这种相关性在两个方面影响了EPS的诊断测试结果。首先，当用作排除参数时，它似乎改善了TMPRSS 2：ERG在EPS中的诊断性能。其次，当用作归一化参数时，它似乎会降低这些相同测试的性能。结论：当比较前列腺癌mRNA诊断的结果时，必须考虑PSA mRNA作为前列腺特异性基因，而不是管家基因。
INTRODUCTION In patients with prostate cancer, luminal prostate-specific antigen (PSA) enters the circulation because the basement membrane and glandular epithelium are damaged. Given that excess mobilization of prostate cells during prostatic massage can influence normalization in diagnostic testing, we studied PSA mRNA levels in expressed prostatic secretions (EPS) from patients undergoing biopsy for prostate cancer to determine if prostate cells are preferentially mobilized from patients with prostate cancer during prostatic massage. MATERIALS AND METHODS Quantitative Reverse-Transcription PCR (qRT-PCR) was used to measure the RNA levels of GAPDH, PSA, TMPRSS2:ERG and PCA3 in EPS specimens obtained from patients undergoing biopsy for prostate cancer. RESULTS The level of PSA mRNA is significantly elevated in EPS specimens obtained from patients with a subsequent diagnosis of prostate cancer. This correlation influenced diagnostic testing results from EPS in two ways. First, when used as an exclusion parameter it appears to improve the diagnostic performance of TMPRSS2:ERG in EPS. Second, when used as a normalization parameter it appears to decrease the performance of these same tests. CONCLUSION When comparing the results of mRNA based prostate cancer diagnostics in EPS it will be essential to consider PSA mRNA as a prostate specific gene and not a housekeeping gene.