INTRINSIC CIRCUIT ORGANIZATION OF THE MAJOR LAYERS AND SUBLAYERS OF THE DORSOLATERAL PREFRONTAL CORTEX IN THE RHESUS-MONKEY
INTRINSIC CIRCUIT ORGANIZATION OF THE MAJOR LAYERS AND SUBLAYERS OF THE DORSOLATERAL PREFRONTAL CORTEX IN THE RHESUS-MONKEY
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DOI：
10.1002/cne.903590109
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发表时间：
1995-08-14
影响因子：
2.5
通讯作者：
GOLDMANRAKIC, PS
中科院分区：
文献类型：
--
作者：
KRITZER, MF;GOLDMANRAKIC, PS
内在连接可能在前额叶联想皮层的认知信息加工中起重要作用。为了深入了解这些回路的组织，我们使用霍乱毒素（b亚基）与胶体金结合，在猕猴的Walker’s区9和46逆行标记了主要层流和层下分裂的皮层内连接。放置在特定皮层层内的显微注射产生了独特的逆行标记。在第II/III层注射产生标记，标记在颗粒上层横向分布（2- 7mm），在第IV-VI层更窄聚焦，即符合柱状结构。相反，与IV层和Vb层相关的局部电路显示出规则的圆柱形组织，而在III层和Va层中，Va层的固有连接是横向广泛的（3-5 mm）。最后，在VI层中注射会产生一个狭窄的细胞标记柱，穿过所有层。前额叶皮层的固有连接排列在中外侧细长的条纹中，这些条纹通常在内侧或外侧方向上不对称分布。此外，这些中侧向定向场中的标记细胞经常分组在离散簇或窄带中。本研究中发现的内在联系不同于初级视觉皮层相应层的局部回路；前额叶皮层独特的内在接线图可能与其特殊的认知和记忆功能有关。(C) 1995 Wiley-Liss, Inc。
Intrinsic connections are likely to play important roles in cognitive information processing in the prefrontal association cortex. To gain insight into the organization of these circuits, intracortical connections of major laminar and sublaminar divisions were retrogradely labeled in Walker's area 9 and 46 in rhesus monkeys by using cholera toxin (B-subunit) conjugated to colloidal gold. Microinjections placed within particular cortical laminae produced unique patterns of retrograde labelinl:. Injections in layers II/III yielded labeling which was laterally widespread (2-7 mm) in supragranular layers, and more narrowly focused, i.e., conforming to a column, in layers IV-VI. In contrast, local circuits associated with layers IV and Vb displayed a regular, cylindrical organization, whereas intrinsic connections of layer Va were laterally extensive (3-5 mm) in layers III and Va. Finally, injections in layer VI gave rise to a narrow column of cell labeling traversing all layers, augmented by laterally extensive labeling (similar to 7 mm) in layer VI. The intrinsic connections of the prefrontal cortex were arrayed within mediolaterally elongated stripes which were often distributed asymmetrically in either the medial or lateral direction. In addition, labeled cells within these mediolaterally oriented fields were frequently grouped within discrete clusters or narrow bands. The intrinsic connections identified in this study differ from the local circuits of corresponding layers reported for primary visual cortex; the unique intrinsic wiring diagram of the prefrontal cortex may be related to its specialized cognitive and mnemonic functions. (C) 1995 Wiley-Liss, Inc.