Medaka double mutants for color interfere and leucophore free:: characterization of the xanthophore-somatolactin relationship using the leucophore free gene
Medaka double mutants for color interfere and leucophore free:: characterization of the xanthophore-somatolactin relationship using the leucophore free gene
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DOI：
10.1007/s00427-005-0040-9
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发表时间：
2006-03-01
影响因子：
2.4
通讯作者：
Mitani, H
中科院分区：
文献类型：
--
作者：
Fukamachi, S;Wakamatsu, Y;Mitani, H
生长激素（SL）在青鳉的体色调节中起重要作用，由颜色干扰（ci）位点编码。ci突变鱼具有组成性增加的数量的leucophores和可见的黄色素随之减少。然而，SL对这些细胞类型的作用机制，以及SL在其他物种的体色调节中的作用尚不清楚。在这项研究中，我们验证了SL-xanthophore的关系，在CI突变鱼使用leucophore免费（LF）基因。对lf幼虫的组织学观察表明，这些突变体不具有分化的白细胞体。ci-lf双突变体，其基因型被确认使用DNA标记，缺乏leucophores;然而，xanthophores的数量仍然很低，表明leucophores是不必要的介导SL信号的xanthophores。这一发现表明一个保守的功能，SL的黄色素调节跨物种，而不是一个青斑特定的和白细胞依赖的SL在体色调节的作用的演变。我们的研究结果还表明，lf基因在leucophore发展上位SL信号传导中具有不可或缺的作用。lf基因还没有被克隆。本研究构建的lf位点周围的高分辨率重组图谱，以及即将公布的青鳉全基因组序列，将允许通过正向遗传学方法快速克隆lf基因。
Somatolactin (SL) plays an essential role in body-color regulation in medaka and is encoded by the color interfere (ci) locus. The ci mutant fish possess constitutively increased numbers of leucophores and a concomitant decrease in visible xanthophores. However, the mechanism of action of SL on these cell types, and the role of SL in body-color regulation in other species, is unknown. In this study, we verified an SL-xanthophore relationship in ci mutant fish using the leucophore free (lf) gene. Histological observation of lf larvae indicated that these mutants do not possess differentiated leucophores. The ci-lf double mutant, whose genotype was confirmed using DNA markers, lacked leucophores; however, the number of xanthophores remained low, demonstrating that leucophores are not necessary for mediating SL signaling to xanthophores. This finding suggests a conserved function for SL in xanthophore regulation across species, rather than the evolution of a medaka-specific and leucophore-dependent role of SL in body-color regulation. Our results also demonstrate that the lf gene has an indispensable role in leucophore development epistatic to SL signaling. The lf gene has not been cloned. The high-resolution recombination map surrounding the lf locus constructed in this study, together with medaka whole genome sequences that will be released soon, will allow the rapid cloning of the lf gene by forward genetic approaches.