MUC8 mucin gene up-regulation in chronic rhinosinusitis
MUC8 mucin gene up-regulation in chronic rhinosinusitis
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DOI：
10.1177/000348940411300812
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发表时间：
2004-08-01
影响因子：
1.4
通讯作者：
Hwang, SJ
中科院分区：
文献类型：
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作者：
Lee, HM;Kim, DH;Hwang, SJ
导致慢性鼻窦炎粘液高分泌的主要机制尚不清楚。本研究旨在探讨MUC8信使核糖核酸(MUC8)及其蛋白在正常鼻窦粘膜和慢性炎症鼻窦粘膜中的表达，并比较MUC8在正常和慢性炎症鼻窦粘膜中的表达。10名接受功能性鼻内窥镜鼻窦手术的慢性鼻窦炎患者作为研究对象。并以10例无鼻窦疾病证据的患者作为对照。提取鼻窦黏膜RNA，半定量逆转录聚合酶链式反应检测MUC8。免疫组织化学分析MUC8蛋白的定位。在人的鼻窦粘膜中检测到编码MUC8的信使RNA。慢性鼻窦炎组织中MUC8基因的表达水平明显高于正常上颌窦黏膜组织。我们发现MUC8蛋白在慢性鼻窦炎鼻窦中的表达比在正常鼻窦黏膜中更强。提示MUC8可能在慢性鼻窦炎鼻窦高分泌的发病机制中起重要作用。
The primary mechanisms leading to mucus hypersecretion in chronic sinus inflammation are not well understood. This study aims to investigate the expression of MUC8 messenger RNA (mRNA) and protein and to compare between normal and chronically inflamed sinus mucosae in terms of the expression of MUC8 mRNA. Ten patients with chronic rhinosinusitis who were undergoing functional endoscopic sinus surgery were recruited for the study. Ten patients with no evidence of sinus disease were used as control subjects. RNAs were extracted from sinus mucosa, and semi quantitative reverse transcription-polymerase chain reaction was performed for MUC8. Localization of MUC8 protein was sought by immunohistochemical analysis. Messenger RNA encoding MUC8 was detected in human sinus mucosa. The level of MUC8 mRNA in chronic rhinosinusitis was significantly increased compared with that in normal maxillary sinus mucosa. We found more intense expression of MUC8 protein in the sinuses with chronic rhinosinusitis than in normal sinus mucosa. These results suggest that MUC8 may play an important role in the pathogenesis of sinus hypersecretion in chronic rhinosinusitis.