Unexpected morphological and molecular diversity of trematode (Haploporidae: Forticulcitinae) parasites of mullets from the ocean Pacific coasts in Middle America
Unexpected morphological and molecular diversity of trematode (Haploporidae: Forticulcitinae) parasites of mullets from the ocean Pacific coasts in Middle America
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DOI：
10.1007/s00436-020-06983-y
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发表时间：
2020-11-28
影响因子：
2
通讯作者：
Garcia-Varela, M.
中科院分区：
文献类型：
--
作者：
Andrade-Gomez, L.;Garcia-Varela, M.
报道了采自中美洲墨西哥、危地马拉和哥斯达黎加的白色乌鱼（Mugil curema）和平头灰乌鱼（Mugil cephalus）的内脏标本中的2个新属和4个新种。新属Ekuarhuni n. gen.和Overstreetoides n. gen.必须建立以容纳两个新物种，Ekuarhuni papillatum n. sp.和Overstreetoides pacificus n.例如，具有独特的形态特征，将它们与先前描述的两个属区分开来。此外，还报道了2个新种，即微小蚁Forticulcita minuta n. sp.和异枝芹Forticulcita isabenn.例如，描述了其特征在于表现出小的体型（< 1100 μ m长）。这些新种与其他三种Forticulcita同属种一起被归类为小型形态型。小叉角蝉sp.的特征在于是属内最小的物种（< 305 μ m）。与此同时，Forticulcita isabababeln。sp.的区别在于其身体大小和精巢长度。对这4个新种的标本进行了核rDNA大亚基（LSU）和内转录间隔区2（ITS2）两个分子标记的测序，并与GenBank中的其他序列进行了比较。系统发育分析与两个核分子标记（LSU + ITS2）的组合数据集内的所有分析物种的分支Forticulcitinae强大的自助支持（100%）和贝叶斯后验概率（1.0）。这四个新物种在其最终宿主中表现出丰度的差异，并广泛分布于中美洲墨西哥、危地马拉和哥斯达黎加的太平洋沿岸沿着。
Two new genera and four new species from subfamily Forticulcitinae are described from the intestines of white mullet (Mugil curema) and flathead grey mullet (Mugil cephalus) collected in 27 localities across a wide geographical range on Pacific Ocean slopes comprising three countries in Middle America: Mexico, Guatemala and Costa Rica. The new genera Ekuarhuni n. gen. and Overstreetoides n. gen. had to be erected to accommodate two new species, Ekuarhuni papillatum n. sp. and Overstreetoides pacificus n. sp., with unique morphological traits that differentiate them from the two genera described previously. In addition, two new species, Forticulcita minuta n. sp. and Forticulcita isabelae n. sp., were described, which were characterized as exhibiting a small body size (< 1100 mu m long). These new species were classified as the diminutive morphotype, together with three other congeneric species of Forticulcita. Forticulcita minuta n. sp. is distinguished by being the smallest species within the genus (< 305 mu m). Meanwhile, Forticulcita isabelae n. sp. is distinguished by its body size and testis length. In specimens of the four new species, sequencing was performed with two molecular markers, the large subunit (LSU) and the internal transcribed spacer 2 (ITS2) of nuclear rDNA, and the results were compared with other sequences available in GenBank. Phylogenetic analyses performed with the combined dataset of the two nuclear molecular markers (LSU + ITS2) placed all the analysed species within the clade of Forticulcitinae with strong bootstrap support (100%) and a high Bayesian posterior probability (1.0). The four new species showed differences in abundance in their definitive hosts and were widely distributed along the Pacific Ocean coasts of Mexico, Guatemala and Costa Rica, in Middle America.