ENERGY-METABOLISM IN LATE PREIMPLANTATION RAT EMBRYOS
ENERGY-METABOLISM IN LATE PREIMPLANTATION RAT EMBRYOS
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DOI：
10.1530/jrf.0.0930245
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发表时间：
1991-09-01
期刊：
影响因子：
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通讯作者：
LEESE, HJ
中科院分区：
文献类型：
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作者：
BRISON, DR;LEESE, HJ
使用非侵入性技术测量单个植入前胚胎的丙酮酸和葡萄糖的消耗以及乳酸的产生。 从交配后第4天至第6天，将胚胎在300-500-nl微滴中培养8-12 h，此时它们从8细胞期发育为扩张的囊胚。 丙酮酸是主要的底物在8细胞/桑椹胚阶段，葡萄糖的摄取超过丙酮酸后，发病的囊胚腔形成。 乳酸的产生与葡萄糖的消耗平行增加。 对于大多数阶段，大约100%的葡萄糖摄取是由乳酸产生的，在某些情况下，必须假设乳酸的额外来源。 体外培养对乳酸产生的影响不大，尽管与新鲜囊胚相比，观察到较低的代谢水平。 大鼠胚胎能够在没有葡萄糖的情况下发育成囊胚，此时乳酸的产生大大减少。
The consumption of pyruvate and glucose, and the production of lactate, by single preimplantation embryos, was measured using a noninvasive technique. Embryos were cultured in 300-500-nl microdrops, for 8-12 h at a time, from Day 4 to Day 6 after mating, when they developed from the 8-cell stage to expanded blastocyst. Pyruvate was the predominant substrate at the 8-cell/morula stage; glucose uptake exceeded that of pyruvate after the onset of blastocoel formation. Lactate production increased in parallel with glucose consumption. For most stages, approximately 100% of the glucose uptake was accountable for by lactate production and in some cases an additional source of lactate must be postulated. Culture in vitro had little effect on lactate production, although a lower level of metabolism was observed compared with fresh blastocysts. Rat embryos were capable of developing to blastocysts in the absence of glucose, when lactate production was greatly reduced.