CYTOSINE METHYLATION INHIBITS REPLICATION OF AFRICAN CASSAVA MOSAIC-VIRUS BY 2 DISTINCT MECHANISMS
CYTOSINE METHYLATION INHIBITS REPLICATION OF AFRICAN CASSAVA MOSAIC-VIRUS BY 2 DISTINCT MECHANISMS
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DOI：
10.1093/nar/21.15.3445
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发表时间：
1993-07-25
影响因子：
14.9
通讯作者：
PASZKOWSKI, J
中科院分区：
文献类型：
--
作者：
ERMAK, G;PASZKOWSKI, U;PASZKOWSKI, J
染色体外复制的病毒DNA通常不含胞嘧啶甲基化，并且当用于复制测定时，体外甲基化的病毒模板是较差的底物。以非洲木薯花叶病毒DNA A为模型，研究了DNA甲基化抑制病毒复制的机制。我们已经构建了两个组件辅助系统，其允许从复制抑制由于复制复合物和甲基化的病毒DNA之间的相互作用改变的复制所必需的病毒基因的转录抑制分离。我们的研究结果表明，甲基化介导的病毒复制减少是由于这两种抑制机制，这提供了两个独立的选择压力，在感染细胞中的甲基化无复制子的维护。
Extrachromosomally replicating viral DNA is usually free of cytosine methylation and viral templates methylated in vitro are poor substrates when used in replication assays. We have investigated the mechanism of inhibition of viral replication by DNA methylation using as a model the DNA A of African cassava mosaic virus. We have constructed two component helper systems which allow for separation of the transcriptional inhibition of viral genes necessary for replication from replication inhibition due to altered interaction between the replication complex and methylated viral DNA. Our results suggest that methylation-mediated reduction of viral replication is due to both repression mechanisms and that this provides two independent selection pressures for the maintenance of methylation-free replicons in infected cells.