Neuron-like differentiation of adipose tissue-derived stromal cells and vascular smooth muscle cells
Neuron-like differentiation of adipose tissue-derived stromal cells and vascular smooth muscle cells
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DOI：
10.1111/j.1432-0436.2006.00081.x
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发表时间：
2006-12-01
期刊：
影响因子：
2.9
通讯作者：
Lin, Ching-Shwun
中科院分区：
文献类型：
--
作者：
Ning, Hongxiu;Lin, Guiting;Lin, Ching-Shwun
脂肪组织来源的基质细胞（ADSC）先前已被证明具有干细胞特性，如转分化和自我更新。由于未来的临床应用可能以自体方式使用这些成体干细胞，因此我们希望建立和表征大鼠ADSC用于临床前试验。在本研究中，我们发现大鼠ADSC在第1代表达干细胞标志物CD 34和STRO-1，但在第3代仅表达STRO-1。这些细胞也可以被诱导分化成脂肪细胞、平滑肌细胞和神经元样细胞，后者表达神经元标记物S100、巢蛋白和NF 70。异丁基甲基黄嘌呤（IBMX），吲哚美辛（INDO），和胰岛素的活性成分在以前建立的神经诱导培养基（NIM），然而，在这里，我们表明，IBMX单独是有效的NIM在诱导形态学变化以及神经元标记物的表达。最后，我们发现血管平滑肌细胞也可以被NIM或IBMX诱导分化为表达NF 70的神经元样细胞。
Adipose tissue-derived stromal cells (ADSC) have previously been shown to possess stem cell properties such as transdifferentiation and self-renewal. Because future clinical applications are likely to use these adult stem cells in an autologous fashion, we wished to establish and characterize rat ADSC for preclinical tests. In the present study, we showed that rat ADSC expressed stem cell markers CD34 and STRO-1 at passage 1 but only STRO-1 at passage 3. These cells could also be induced to differentiate into adipocytes, smooth muscle cells, and neuron- like cells, the latter of which expressed neuronal markers S100, nestin, and NF70. Isobutylmethylxanthine (IBMX), indomethacin (INDO), and insulin were the active ingredients in a previously established neural induction medium (NIM); however, here we showed that IBMX alone was as effective as NIM in the induction of morphological changes as well as neuronal marker expression. Finally, we showed that vascular smooth muscle cells could also be induced by either NIM or IBMX to differentiate into neuron-like cells that expressed NF70.