Factors Affecting the Measurement of Enzymatic Hydrolysate Gross Energy of Corn and Soybean Meal Using Simulative Digestion System
Factors Affecting the Measurement of Enzymatic Hydrolysate Gross Energy of Corn and Soybean Meal Using Simulative Digestion System
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发表时间：
2012
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通讯作者：
Zhang Hongfu
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作者：
Zhang Hongfu
本实验对样品粒度、未消化残渣洗涤次数、处理后透析袋的保存时间影响干物质消化率（DMD）和酶解产物总能量（EHGE）的测定本研究建立了一种基于模拟消化系统的体外消化方法，对玉米和豆粕的EHGE进行了测定，为建立一种基于模拟消化系统的饲料EHGE测定方法提供了参考。采用单因素完全随机试验设计，设11个处理，每个处理5个重复，每个重复1个消化管，分别研究了处理1- 3、4 -7和8-11的样品粒度、未消化残渣洗涤次数和保存时间对玉米和豆粕DMD和EHGE的影响。1）样品粒度显著影响玉米和豆粕的DMD和EHGE当粒度从40目减小到100目时，玉米的DMD和EHGE均增大，其中40目到60目的变化更明显，而60目的变化更明显。粒度从40目减小到100目时，60目豆粕的DMD和EHGE均高于40目和100目豆粕，未消化残渣的洗涤次数对玉米和豆粕的DMD和EHGE有显著影响玉米和豆粕的DMD和EHGE随未消化残渣洗涤次数的增加而趋于稳定，洗涤6次以上后，玉米和豆粕的DMD和EHGE的变化均在模拟消化系统的系统误差允许范围内; 3）处理后的透析袋保存时间对玉米和豆粕的DMD和EHGE也有显著影响而在4个处理中，玉米和豆粕的DMD和EHGE的差异分别不超过0.94%和0.37 MJ/kg，均在模拟消化系统的系统误差允许范围内。用该方法测定饲料中EHGE的适宜条件是样品粒度为60目，未消化残渣洗涤6次经处理的透析袋在4 ℃下保存90 d对玉米和豆粕的DMD和EHGE无明显影响。
This experiment was conducted to study on sample particle size,wash times of undigested residues,and preservation time of treated dialysis bag affecting the determination of dry matter digestibility(DMD) and enzymatic hydrolysate gross energy(EHGE) of corn and soybean meal using an in vitro digestion method based on simulative digestion system.It would provide a reference to develop a method for measuring the EHGE of feed using simulative digestion system.A single factor completely randomized design with 11 treatments was adapted.Each treatment contained 5 replicates with 1 digestion tube per replicate.The treatments 1-3,4-7 and 8-11 were used to determine the effects of sample particle size,wash times of undigested residues,and preservation time on DMD and EHGE of corn and soybean meal,respectively.The results showed as follows: 1) sample particle size significantly affected the DMD and EHGE of corn and soybean meal(P0.05).When particle size was decreased from 40 mesh to 100 mesh,the DMD and EHGE of corn were increased.The changes of DMD and EHGE of corn were both more obvious in particle size ranged from 40 mesh to 60 mesh.However,when particle size was decreased from 40 mesh to 100 mesh,the DMD and EHGE of soybean meal with 60 mesh were higher than those of the samples with 40 mesh and 100 mesh.And the difference of DMD of soybean meal with 40,60 and 100 mesh was not more than 0.70%.2) The wash times of undigested residues significantly affected the DMD and EHGE of corn and soybean meal(P0.05).The DMD and EHGE of corn and soybean meal became stable when increasing wash times of undigested residues.After washing undigested residues for above 6 times,the variations of DMD and EHGE of corn and soybean meal both were in the permitted range of systematic error of simulative digestion system.3) The preservation time of treated dialysis bag also significantly affected the DMD and EHGE of corn and soybean meal(P0.05).However,in the 4 treatments,the differences of the DMD and EHGE of corn and soybean meal were not more than 0.94% and 0.37 MJ/kg,respectively,which were in the permitted range of systematic error of simulative digestion system.In conclusion,60 mesh sample particle size and 6 times for washing undigested residues are the suitable condition in determination of EHGE of feed using the in vitro digestion method based on simulative digestion system.Preservation of treated dialysis bag for 90 d and at 4 ℃ don't obviously affect the DMD and EHGE of corn and soybean meal.