Golden-star nanoparticles as adjuvant effectively promotes immune response to foot-and-mouth disease virus-like particles vaccine
Golden-star nanoparticles as adjuvant effectively promotes immune response to foot-and-mouth disease virus-like particles vaccine
复制标题
金星纳米颗粒作为佐剂有效促进口蹄疫病毒样颗粒疫苗的免疫反应
DOI：
10.1016/j.vaccine.2018.09.030
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发表时间：
2018
期刊：
影响因子：
5.5
通讯作者：
Guo Huichen
中科院分区：
文献类型：
--
作者：
Teng Zhidong;Sun Shiqi;Chen Hao;Huang Jie;Du Ping;Dong Hu;Xu Xiaoyu;Mu Suyu;Zhang Zhijun;Guo Huichen
病毒样颗粒（Virus-like particles，VLP）因其安全、易制备和免疫特性而成为现代疫苗研究的热点。为了进一步增强VLP的免疫效果，我们合成并使用金星纳米颗粒（AuSNs）作为疫苗的佐剂。以口蹄疫病毒颗粒（VLP）为靶抗原，结合金纳米颗粒（AuSNs）。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、蛋白质印迹、紫外光吸收和形态测量分析对FMD VLPs-AuSNs复合物进行表征。结果表明，FMD VLPs-AuSNs复合物在不同的细胞系中是无毒的。与单独的FMD VLP相比，AuSNs与FMD VLP组合时可以有效地促进FMD VLP进入细胞并改善巨噬细胞活化。进一步的动物免疫和攻毒试验显示，AuSNs佐剂组的特异性免疫应答和保护率均高于常规矿物油（ISA 206）佐剂组。AuSNs可有效提高口蹄疫病毒性颗粒疫苗的免疫保护效果，并有望成为其他疫苗的新型佐剂。
Virus-like particles (VLPs) have become a hot topic in modern vaccine research because of its safety, facile production, and immune properties. To further enhance the immune effect of VLPs, we synthesized and used gold-star nanoparticles (AuSNs) as adjuvant for vaccine. Foot-and-mouth disease (FMD) VLPs as target antigen were combined with AuSNs. The FMD VLPs-AuSNs complex was characterized through sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blot, ultraviolet light absorption, and morphological measurement analyses. Result indicated that the FMD VLPs-AuSNs complex is non-toxic in different cell lines. AuSNs can effectively promote the entry of FMD VLPs into cells and improve macrophages activation when combined with FMD VLPs compared with FMD VLPs alone. Further animal vaccination and challenge tests revealed that the specific immune response and protection rate of AuSNs adjuvant group is higher than that of conventional mineral oil (ISA206) adjuvant group. AuSNs can effectively improve the immune protection effects of FMD VLPs vaccines, and exhibit potential as a new adjuvant for other vaccines.