The intestinal anti-inflammatory effect of quercitrin is associated with an inhibition in iNOS expression
The intestinal anti-inflammatory effect of quercitrin is associated with an inhibition in iNOS expression
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DOI：
10.1038/sj.bjp.0705941
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发表时间：
2004-12-01
影响因子：
7.3
通讯作者：
Gálvez, J
中科院分区：
文献类型：
--
作者：
Camuesco, D;Comalada, M;Gálvez, J
槲皮素（Quercitrin, 3-rhamnosylquercetin）是一种具有抗氧化特性的生物类黄酮，对实验性结肠炎具有抗炎作用。在本研究中，进行了不同的体内实验，以评估这种作用的作用机制，特别关注其对促炎介质的作用，包括一氧化氮（NO）将右旋糖酐硫酸钠（DSS）掺入饮用水中，诱导雌性Wistar大鼠实验性结肠炎。口服槲皮素（1或5 mg kg（-1）天（-1））治疗结肠炎大鼠，可改善以预防性给药方案给予时引起的炎症过程的演变。当槲皮素(1 mg kg（-1) day(-1)）用于已建立的结肠炎时，它促进了炎症粘膜的恢复槲皮素发挥的有益作用在组织学和生物化学上都得到了证明，并且与结肠氧化状态的改善有关，这是DSS引起的结肠损伤的结果。此外，还观察到结肠NO合成酶活性的降低，这可能与通过下调核因子- kappab的结肠活性来降低该酶的诱导形式表达有关免疫组织化学研究表明，槲皮素处理减少了炎症组织中的巨噬细胞和粒细胞浸润。
I Quercitrin, 3-rhamnosylquercetin, is a bioflavonoid with antioxidant properties, which exerts anti-inflammatory activity in experimental colitis. In the present study, different in vivo experiments were performed in order to evaluate the mechanisms of action involved in this effect, with special attention to its effects on proinflammatory mediators, including nitric oxide (NO).2 Experimental colitis was induced in female Wistar rats by incorporation of dextran sodium sulfate (DSS) in drinking water. Oral treatment of quercitrin (1 or 5 mg kg(-1) day(-1)) to colitic rats ameliorated the evolution of the inflammatory process induced when administered in a preventative dosing protocol. When quercitrin (1 mg kg(-1) day(-1)) was administered on established colitis, it facilitated the recovery of the inflamed mucosa.3 The beneficial effects exerted by quercitrin were evidenced both histologically and biochemically, and were associated with an improvement in the colonic oxidative status, altered as a consequence of the colonic insult induced by DSS. In addition, a reduction of colonic NO synthase activity was observed, probably related to a decreased expression in the inducible form of the enzyme via downregulation in the colonic activity of the nuclear factor-kappaB.4 Immunohistochemical studies showed that quercitrin treatment reduced macrophage and granulocyte infiltration in the inflamed tissue.