Retinoid-dependent restriction of human immunodeficiency virus type 1 replication in monocytes/macrophages.
Retinoid-dependent restriction of human immunodeficiency virus type 1 replication in monocytes/macrophages.
复制标题
类维生素A依赖性限制人类免疫缺陷病毒1型在单核细胞/巨噬细胞中的复制。
DOI：
10.1128/jvi.78.6.2819-2830.2004
复制
发表时间：
2004
影响因子：
5.4
通讯作者：
Viglianti,GregoryA
中科院分区：
文献类型：
--
作者：
Hanley,TimothyM;Kiefer,HeatherLB;Schnitzler,AlettaC;Marcello,JenniferE;Viglianti,GregoryA
Vitamin A deficiency has been correlated with increased severity of human immunodeficiency virus type 1 (HIV-1)-associated disease. Moreover, vitamin A supplementation can reduce AIDS-associated morbidity and mortality. Our group and others have shown that retinoids, the bioactive metabolites of vitamin A, repress HIV-1 replication in monocytic cell lines and primary macrophages by blocking long-terminal-repeat (LTR)-directed transcription. Based on these studies, we hypothesize that retinoids are natural repressors of HIV-1 in vivo. We show here that all-trans-retinoic acid (RA)-mediated repression of HIV-1 activation requires pretreatment for at least 12 h and is blocked by the protein synthesis inhibitors cycloheximide and puromycin. Studies of the kinetics of RA-mediated repression in U1 cells and primary monocyte-derived macrophages (MDMs) reveal that the repressive effects of RA on HIV-1 expression are long-lasting but reversible. We demonstrate that HIV-1 expression is activated when U1 cells or MDMs are cultured in retinoid-free synthetic medium and show that physiological concentrations of RA repress this activation. In addition, the synthetic pan-retinoic acid receptor antagonist BMS-204 493 activates HIV-1 replication in U1 cells in a dose-dependent manner, suggesting that RA-induced transactivation of cellular gene expression is required for HIV-1 repression. Together, these data support the hypothesis that retinoids present in tissue culture media in vitro and serum in vivo maintain HIV-1 in a transcriptionally repressed state in monocytes/macrophages.
