PLASMA + CYTOPLASMIC MEMBRANE FRAGMENTS FROM EHRLICH ASCITES CARCINOMA
PLASMA + CYTOPLASMIC MEMBRANE FRAGMENTS FROM EHRLICH ASCITES CARCINOMA
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DOI：
10.1073/pnas.52.3.721
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发表时间：
1964-01-01
影响因子：
11.1
通讯作者：
KAMAT, VB
中科院分区：
文献类型：
--
作者：
WALLACH, DFH;KAMAT, VB
描述了分析以NaBR、蔗糖和蔗糖聚合物Ficoll为梯度的Ehrlich腹水癌微粒体膜组分浮力密度分布的程序。微粒体膜表现为选择性透过性囊，在静电和渗透的影响下膨胀和收缩。质膜碎片由于其独特的酶学和免疫学特性，可以通过在蔗糖-聚合物梯度中的平衡而与其他膜组分分离。
Procedures are described for the analysis of the buoyant density distributions of the membrane components of Ehrlich ascites carcinoma microsomes in gradients of NaBr, sucrose, and the sucrose polymer Ficoll. The microsomal membranes behave as selectively permeable sacs, which expand and contract under electrostatic and osmotic influences. Plasma membrane fragments, localized by their unique enzymatic and immuno-logic properties, can be separated from the other membrane components by equilibration in the sucrose-polymer gradients.