Preparation of a homogeneous and stable form of bovine milk lipoprotein lipase.
Preparation of a homogeneous and stable form of bovine milk lipoprotein lipase.
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均匀且稳定形式的牛乳脂蛋白脂肪酶的制备。
DOI：
10.1080/10826068508062267
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Jackson,RL
中科院分区：
文献类型：
--
作者：
Socorro,L;Green,CC;Jackson,RL
采用肝素-琼脂糖凝胶柱层析两步法从牛脱脂乳中纯化脂蛋白脂肪酶。如在十二烷基硫酸钠中通过梯度聚丙烯酰胺凝胶电泳所测定的，纯化的脂蛋白脂肪酶显示表观分子量为55，000的单一条带。在洗涤缓冲液中使用Triton N-101是对先前报道的纯化程序的主要改进，该纯化程序导致酶的稳定均质制备。
Lipoprotein lipase was purified to homogeneity from bovine skim milk by a two-step procedure using chromatography on heparin-Sepharose. As determined by gradient-polyacrylamide gel electrophoresis in sodium dodecyl sulfate, purified lipoprotein lipase showed a single band with an apparent molecular weight of 55,000. The use of Triton N-101 in the washing buffers was the major improvement from previously reported purification procedures that resulted in a stable homogeneous preparation of the enzyme.