Expression of FGF receptor gene in rat development.
Expression of FGF receptor gene in rat development.
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DOI：
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发表时间：
1991-02
期刊：
影响因子：
4.6
通讯作者：
A. Wanaka;J. Milbrandt;Eugene M. Johnson
中科院分区：
文献类型：
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作者：
A. Wanaka;J. Milbrandt;Eugene M. Johnson
我们通过原位杂交组织化学检查了大鼠发育过程中 FGF 受体 (FGF-R) mRNA 的表达。检查胚胎组织（E9、E12、E14、E17）和出生后神经组织（P1、P7、P14、成人）。我们在不同发育阶段的各种组织中检测到显着水平的 FGF-R mRNA。正如之前使用其他方法的研究所假设的，FGF-R 基因表达主要在中胚层和神经外胚层衍生的组织中观察到。在神经系统中，基因表达模式受到发育调节。在胚胎中，FGF-R mRNA 主要在中枢神经系统（CNS）的室管膜层中检测到。出生后，在特定神经元群体（例如海马神经元）中观察到 FGF-R 转录本。 FGF-R mRNA 也在感觉系统中发现，例如晚期胚胎的三叉神经节和背根神经节；然而，FGF-R mRNA 在出生后下降。 FGF-R mRNA 表达在发育中的视网膜中受到调节：在胚胎阶段的色素上皮和神经母细胞层中观察到 FGF-R 信息；出生后，在神经节细胞和内颗粒层中发现它们。在非神经元胚胎组织中，多种器官表达 FGF-R 信息。特别是，椎前柱、骨、肾和皮肤显示出高水平的表达。这些观察结果强化了 FGF 对各种组织发育产生影响的观点。
We examined the expression of FGF-receptor (FGF-R) mRNA during rat development with in situ hybridization histochemistry. Embryonic tissues (E9, E12, E14, E17) and postnatal neural tissues (P1, P7, P14, adult) were examined. We detected significant levels of FGF-R mRNA in various tissues at different developmental stages. As postulated by previous studies using other methods, FGF-R gene expression was observed primarily in mesoderm- and neuroectoderm-derived tissues. In the nervous system, the pattern of gene expression was developmentally regulated; in embryos, FGF-R mRNA was mainly detected in the ependymal layer of the central nervous system (CNS). Postnatally, FGF-R transcripts were observed in specific neuronal populations, such as hippocampal neurons. FGF-R mRNA was also found in sensory systems such as trigeminal and dorsal root ganglia in late stage embryos; however, FGF-R mRNA decreased in the postnatal period. FGF-R mRNA expression was modulated in the developing retina: FGF-R messages were observed in the pigment epithelium and neuroblast layer at embryonic stages; in the postnatal period, they were found in the ganglion cell and inner granular layer. In non-neuronal embryonic tissues, a wide variety of organs expressed FGF-R message. Particularly, the prevertebral column, bone, kidney and skin showed high levels of expression. These observations reinforce the idea that FGF exerts effects on the development of various tissues.