Analysis of Pollen Allergens in Lily by Transcriptome and Proteome Data
Analysis of Pollen Allergens in Lily by Transcriptome and Proteome Data
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通过转录组和蛋白质组数据分析百合花粉过敏原
DOI：
10.3390/ijms20235892
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发表时间：
2019-12-01
影响因子：
5.6
通讯作者：
Teng, Nianjun
中科院分区：
文献类型：
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作者：
Feng, Jingxian;Wu, Ze;Teng, Nianjun
百合（Lilium spp.）花药含有大量花粉。百合花粉中是否含有过敏原尚不清楚，因此筛选花粉过敏相关蛋白和基因是必要的。通过显微观察，确定了百合‘西伯利亚’的花粉发育时期。以早期单核小孢子和成熟花粉为测序材料。对花粉转录组的分析鉴定了差异表达的基因（DEG），例如，与花粉过敏原相关的Profilin、Phl p 7（Polcalcin）、Ole e 1和Phl p 11。蛋白质组分析证实花粉变应原蛋白含量显著增加。LoProfilin和LoPolcalcin（注释为过敏原蛋白）的表达水平在成熟花粉中逐渐增加。克隆了LoProfilin和LoPolcalcin基因，其开放阅读框长度分别为396 bp和246 bp，分别编码131和81个氨基酸。氨基酸序列和结构比对结果表明，LoProfilin和LoPolcalcin的蛋白质序列高度保守。亚细胞定位分析显示LoProfilin蛋白定位于细胞质和细胞核。LoProfilin和LoPolcalcin在成熟花粉中在转录和蛋白水平上高度表达。三级结构预测分析确定LoProfilin和LoPolcalcin为百合花粉中的潜在过敏原。
The lily (Lilium spp.) anther contains a lot of pollen. It is not known if lily pollen contains allergens, and therefore screening pollen allergy-related proteins and genes is necessary. The pollen development period of lily 'Siberia' was determined by microscope observation. Early mononuclear microspores and mature pollens were used as sequencing materials. The analysis of the pollen transcriptome identified differentially expressed genes (DEGs), e.g., Profilin, Phl p 7 (Polcalcin), Ole e 1, and Phl p 11, which are associated with pollen allergens. The proteome analysis positively verified a significant increase in pollen allergenic protein content. The expression levels of LoProfiilin and LoPolcalcin, annotated as allergen proteins, gradually increased in mature pollen. LoProfiilin and LoPolcalcin were cloned and their open reading frame lengths were 396 bp and 246 bp, which encoded 131 and 81 amino acids, respectively. Amino acid sequence and structure alignment indicated that the protein sequences of LoProfilin and LoPolcalcin were highly conserved. Subcellular localization analysis showed that LoProfilin protein was localized in the cell cytoplasm and nucleus. LoProfilin and LoPolcalcin were highly expressed in mature pollen at the transcriptional and protein levels. A tertiary structure prediction analysis identified LoProfilin and LoPolcalcin as potential allergens in lily pollen.