Comparison of linear intrascan and interscan dynamic ranges of Orbitrap and ion-mobility time-of-flight mass spectrometers
Comparison of linear intrascan and interscan dynamic ranges of Orbitrap and ion-mobility time-of-flight mass spectrometers
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DOI：
10.1002/rcm.7981
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发表时间：
2017-11-30
影响因子：
2
通讯作者：
Walker, Stephan
中科院分区：
文献类型：
--
作者：
Kaufmann, Anton;Walker, Stephan
基本原理质谱仪的线性扫描内和扫描间动态范围在代谢组和残留分析中非常重要。如果必须在重基质样品中检测和定量低丰度和高丰度离子，则必须采用大的线性动态范围。对现代高分辨率质谱 (HRMS) 提供的这些性能标准进行了系统研究。方法比较包括两代 Orbitraps 和离子淌度四极杆飞行时间 (QTOF) 系统。此外，还研究了所用仪器提供的不同扫描模式。测量涵盖五个数量级浓度范围的不同化合物的校准曲线，以评估线性扫描间动态范围。通过在非常强烈的背景下重复这些测量来评估线性扫描内动态范围和由此产生的质量精度。结果现代 HRMS 仪器可以显示五个数量级的线性动态范围。然而，线性动态范围通常受到检测能力（灵敏度和选择性）和电喷雾电离的限制。与 TOF 仪器相反，Orbitrap 的扫描内动态范围较小。这是由于 C-trap 和 Orbitrap 容量有限。测试的 TOF 仪器显示出比 Orbitraps 更差的质量精度。相比之下，与离子淌度装置联用似乎不会影响线性动态范围。结论现代 HRMS 仪器的线性动态范围已得到显着改善。这也指在高离子丰度下实际上不存在系统质量转移。在分析复杂的基质提取物时，当前 Orbitrap 技术的扫描内动态范围可能仍然是一个限制。另一方面，线性动态范围不仅受到检测器技术的限制，而且还可能被发生离子电离和转移的外围设备缩短。
RationaleThe linear intrascan and interscan dynamic ranges of mass spectrometers are important in metabolome and residue analysis. A large linear dynamic range is mandatory if both low- and high-abundance ions have to be detected and quantitated in heavy matrix samples. These performance criteria, as provided by modern high-resolution mass spectrometry (HRMS), were systematically investigated.MethodsThe comparison included two generations of Orbitraps, and an ion mobility quadrupole time-of-flight (QTOF) system In addition, different scan modes, as provided by the utilized instruments, were investigated. Calibration curves of different compounds covering a concentration range of five orders of magnitude were measured to evaluate the linear interscan dynamic range. The linear intrascan dynamic range and the resulting mass accuracy were evaluated by repeating these measurements in the presence of a very intense background.ResultsModern HRMS instruments can show linear dynamic ranges of five orders of magnitude. Often, however, the linear dynamic range is limited by the detection capability (sensitivity and selectivity) and by the electrospray ionization. Orbitraps, as opposed to TOF instruments, show a reduced intrascan dynamic range. This is due to the limited C-trap and Orbitrap capacity. The tested TOF instrument shows poorer mass accuracies than the Orbitraps. In contrast, hyphenation with an ion-mobility device seems not to affect the linear dynamic range.ConclusionsThe linear dynamic range of modern HRMS instrumentation has been significantly improved. This also refers to the virtual absence of systematic mass shifts at high ion abundances. The intrascan dynamic range of the current Orbitrap technology may still be a limitation when analyzing complex matrix extracts. On the other hand, the linear dynamic range is not only limited by the detector technology, but can also be shortened by peripheral devices, where the ionization and transfer of ions take place.