Induction of heme oxygenase mRNA by cobalt protoporphyrin in rat liver.
Induction of heme oxygenase mRNA by cobalt protoporphyrin in rat liver.
复制标题
钴原卟啉在大鼠肝脏中诱导血红素加氧酶 mRNA。
DOI：
10.1016/0304-4165(91)90206-v
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Drummond,GS
中科院分区：
文献类型：
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作者：
Smith,TJ;Haque,S;Drummond,GS
在成年雄性大鼠肝脏中，研究了钴（III）-原卟啉对血红素加氧酶活性和mRNA含量的影响。钴原卟啉（25 μmol/kg体重）可增强血红素加氧酶（血红素降解的限速酶）的活性，并呈时间依赖性。给药后8-16 h，酶活性水平增加2倍，48 h时比基线值高6倍。给药钴原卟啉导致肝脏中血红素加氧酶mRNA显著增加。处理后2小时内，mRNA水平增加了7.9倍。血红素加氧酶mRNA的诱导在8小时达到最大，此时水平为基线的58.5倍。在每个测试的时间点，血红素加氧酶mRNA的增加是酶活性的几倍。
The effect of cobaltic(III)-protoporphyrin on heme oxygenase activity and mRNA content was examined in vivo in the adult male rat liver. The activity of heme oxygenase, the rate-limiting enzyme in the degradation of heme, was enhanced, as expected, by cobalt protoporphyrin (25 μmol/kg body weight) in a time-dependent manner. Levels of enzyme activity were increased 2-fold by 8–16 h following treatment and were 6-fold higher than baseline values by 48 h. Administration of cobalt protoporphyrin resulted in a marked increase in heme oxygenase mRNA in the liver. Within 2 h of treatment, mRNA levels had increased 7.9-fold. The induction of heme oxygenase mRNA was maximal at 8 h when the levels were 58.5-fold above baseline. At every time point tested, the increase in heme oxygenase mRNA was several fold greater than that of enzyme activity.