gridlock, an HLH gene required for assembly of the aorta in zebrafish
gridlock, an HLH gene required for assembly of the aorta in zebrafish
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DOI：
10.1126/science.287.5459.1820
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发表时间：
2000-03-10
期刊：
影响因子：
56.9
通讯作者：
Fishman, MC
中科院分区：
文献类型：
--
作者：
Zhong, TP;Rosenberg, M;Fishman, MC
脊椎动物胚胎的第一动脉和静脉通过成血管细胞的迁移和合并在躯干中聚集形成内皮管。斑马鱼的gridlock（grl）突变选择性地扰乱动脉（主动脉）的组装。这里显示grl编码碱性螺旋-环-螺旋（bHLH）蛋白，其属于bHLH蛋白分裂家族的Hairy/增强子。grl基因在血管形成之前在侧板中胚层中表达，之后在主动脉中表达而在静脉中不表达。这些结果表明，动脉内皮细胞的身份，甚至建立在血流的开始，并牵连grl基因在分配血管特异性细胞的命运。
The first artery and vein of the vertebrate embryo assemble in the trunk by migration and coalescence of angioblasts to form endothelial tubes. The gridlock (grl) mutation in zebrafish selectively perturbs assembly of the artery (the aorta). Here it is shown that grl encodes a basic helix-loop-helix (bHLH) protein belonging to the Hairy/Enhancer of the split family of bHLH proteins. The grl gene is expressed in Lateral plate mesoderm before vessel formation, and thereafter in the aorta and not in the vein. These results suggest that the arterial endothelial identity is established even before the onset of blood flow and implicate the grl gene in assignment of vessel-specific cell fate.