In situ control of root-bacteria interactions using optical trapping in transparent soil.
In situ control of root-bacteria interactions using optical trapping in transparent soil.
复制标题
使用透明土壤中的光捕获原位控制根-细菌相互作用。
DOI：
10.1093/jxb/erac437
复制
发表时间：
2023
影响因子：
6.9
通讯作者：
Ge S
中科院分区：
文献类型：
--
作者：
Ge S
细菌在根表面的附着是病原体定殖或内化以及随后感染植物之前的重要步骤。不幸的是，细菌附着还没有得到很好的理解，因为这种现象很难观察。在这里，我们评估是否可以克服这种限制，使用光学捕获的方法。我们已经开发了一个系统的基础上对向传播的光束和研究其能力，引导黑腐果胶杆菌（Pba）细胞不同的根细胞类型的间隙内的透明土壤。细菌细胞被成功地捕获并引导到根毛细胞、表皮细胞、边缘细胞和激光消融损伤的组织。最后，我们使用该系统来量化可逆附着后牙根表面上的Pba细胞的细菌细胞脱离率。光学捕获技术可以大大提高我们的能力，确定性地表征机制连接到附着和形成的根际生物膜。
Bacterial attachment on root surfaces is an important step preceding the colonization or internalization and subsequent infection of plants by pathogens. Unfortunately, bacterial attachment is not well understood because the phenomenon is difficult to observe. Here we assessed whether this limitation could be overcome using optical trapping approaches. We have developed a system based on counter-propagating beams and studied its ability to guidePectobacterium atrosepticum(Pba) cells to different root cell types within the interstices of transparent soils. Bacterial cells were successfully trapped and guided to root hair cells, epidermal cells, border cells, and tissues damaged by laser ablation. Finally, we used the system to quantify the bacterial cell detachment rate of Pba cells on root surfaces following reversible attachment. Optical trapping techniques could greatly enhance our ability to deterministically characterize mechanisms linked to attachment and formation of biofilms in the rhizosphere.