Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)
Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)
复制标题
PFOS 和 PFOA 在原代培养的淡水罗非鱼（Oreochromis niloticus）肝细胞中诱导氧化应激和细胞凋亡
DOI：
10.1016/j.aquatox.2007.02.006
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发表时间：
2007-05-01
影响因子：
4.5
通讯作者：
Zhou, Bingsheng
中科院分区：
文献类型：
--
作者：
Liu, Chunsheng;Yu, Ke;Zhou, Bingsheng
全氟有机化合物（pfos）是广泛存在于环境、野生动物和人类中的新型持久性有机污染物。研究了全氟辛烷磺酸（PFOS）和全氟辛酸（PFOA）对淡水罗非鱼（Oreochromis niloticus）原代培养肝细胞氧化应激和诱导凋亡的细胞毒理学作用。将培养的肝细胞分别暴露于PFOS或PFOA（0、1、5、15和30 mg L-1）中24 h，用台盼蓝排除法测定细胞活力下降的剂量依赖性。活性氧（ROS）诱导显著，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）活性升高，谷胱甘肽过氧化物酶（GPx）和谷胱甘肽s转移酶（GST）活性降低。经PFOA和PFOS处理后，谷胱甘肽（GSH）含量降低。脂质过氧化（LPO）水平（以马来二醛，MDA测量）的剂量依赖性增加仅在PFOA暴露组中观察到，而LPO在PFOS暴露组中保持不变。此外，在全氟辛烷磺酸和全氟辛酸暴露组中，caspase-3, -8， -9活性明显活化。琼脂糖凝胶电泳进一步表征了典型的DNA断裂（DNA阶梯）。综上所述，PFOS和PFOA能够在原代培养的罗非鱼肝细胞中产生氧化应激，诱导半胱天冬酶参与细胞凋亡。(c) 2007 Elsevier B.V.版权所有
Perfluorinated organic compounds (PFOCs) are emerging persistent organic pollutants (POPs) widely present in the environment, wildlife and human. We studied the cellular toxicology of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) on oxidative stress and induction of apoptosis in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Cultured hepatocytes were exposed to PFOS or PFOA (0, 1, 5, 15 and 30 mg L-1) for 24 h, and a dose-dependent decrease in cell viability was determined using trypan blue exclusion method. Significant induction of reactive oxygen species (ROS) accompanied by increases in activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were found, while activities of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were decreased. Glutathione (GSH) content was reduced following treatment of PFOA and PFOS. A dose-dependent increase in the lipid peroxidation (LPO) level (measured as maleic dialdehyde, MDA) was observed only in the PFOA exposure groups, whereas LPO remained unchanged in the PFOS exposure groups. Furthermore, a significant activation of caspase-3, -8, -9 activities was evident in both PFOS and PFOA exposure groups. Typical DNA fragmentation (DNA laddering) was further characterized by agarose gel electrophoresis. The overall results demonstrated that PFOS and PFOA are able to produce oxidative stress and induce apoptosis with involvement of caspases in primary cultured tilapia hepatocytes. (c) 2007 Elsevier B.V. All rights reserved.