Upregulation of Wilms' tumour gene 1 (WT1) in uterine sarcomas
Upregulation of Wilms' tumour gene 1 (WT1) in uterine sarcomas
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DOI：
10.1016/j.ejca.2007.04.008
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发表时间：
2007-07-01
影响因子：
8.4
通讯作者：
Amant, F.
中科院分区：
文献类型：
--
作者：
Coosemans, A.;Nik, S. Amini;Amant, F.
目的：Wilms‘s肿瘤基因(WT1)在多种肿瘤中的过表达已被证实。我们课题组先前对子宫平滑肌肉瘤(LMS)和癌肉瘤(CS)细胞周期的研究表明WT1可能起作用。方法：收集子宫肉瘤27例，子宫肌瘤38例，子宫内膜间质肉瘤15例，未分化肉瘤7例。用免疫组织化学(IHC)法检测87例标本中WT1的表达，随机抽取23例标本用RT-PCR法检测m-RNA表达，12例标本用免疫印迹法检测WT1的表达，分离细胞质和核蛋白。结果：IHC在12/27例CS、29/38例LMS、7115例ESS和4/7 US中检测到WT1阳性。除一个样本外，所有样本的RT-PCR均为阳性。免疫印迹均为阳性，9/12例有较多胞浆表达。结论：WT1在子宫肉瘤中高表达。由于增加的mRNA水平决定了生物学作用，WT1可能对子宫肉瘤的生物学作用有贡献。(C)2007爱思唯尔有限公司。保留所有权利。
Aim: Overexpression of Wilms' tumour gene (WT1) has been proven in several tumours. Previous research of our group on the cell cycle of uterine leiomyosarcoma (LMS) and carcinosarcoma (CS) suggested a possible role for WT1. We therefore intended to further explore the expression pattern of \WT1 in uterine sarcomas.Methods: 27 CS, 38 LMS, 15 endometrial stromal sarcomas (ESS) and seven undifferentiated sarcomas (US) were collected. WT1 expression was evaluated by immunohistochemistry (IHC) in 87 samples, by RT-PCR (m-RNA expression) in 23 random selected samples and by Western blotting in 12 samples, separating cytoplasmic and nuclear proteins. A pilot study to detect mutations (exons 7-10) was performed on eight samples.Results: IHC showed WT1 positivity in 12/27 CS, 29/38 LMS, 7115 ESS and 4/7 US. All-but-one sample had a positive RT-PCR. All Western blottings were positive with more cytoplasmic expression in 9/12 cases. No mutations were found.Conclusions: WT1 is overexpressed in uterine sarcomas. Since increased levels of mRNA determine the biological role, WT1 might contribute to uterine sarcoma tumour biology. (C) 2007 Elsevier Ltd. All rights reserved.