The Tobacco Mosaic Virus Origin of Assembly Sequence is Dispensable for Specific Viral RNA Encapsidation but Necessary for Initiating Assembly at a Single Site.
The Tobacco Mosaic Virus Origin of Assembly Sequence is Dispensable for Specific Viral RNA Encapsidation but Necessary for Initiating Assembly at a Single Site.
复制标题
烟草花叶病毒装配序列的起源对于特定病毒 RNA 衣壳化来说是可有可无的，但对于在单个位点启动装配是必需的。
DOI：
10.1016/j.jmb.2022.167873
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发表时间：
2022
影响因子：
5.6
通讯作者：
Saunders K
中科院分区：
文献类型：
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作者：
Saunders K
我们研究了烟草花叶病毒（TMV）组装序列起源（OAS）的存在是否对复制病毒RNA的特异性封装是必要的。为此，在有或没有绿洲植物的情况下，通过复制RNA构建物来表达TMV外壳蛋白。在这两种情况下，复制的RNA都被特异性地封装以产生核蛋白纳米棒，尽管在没有OAS的情况下产量降低到有OAS时的60%左右。此外，在没有OAS的情况下产生的纳米棒在长度上更加不均匀，并且包含频繁的结构不连续。这些结果强烈表明，OAS的功能是为病毒组装的起始提供一个独特的位点，导致一次启动螺旋，而不是选择病毒RNA进行包装。
We have investigated whether the presence of the origin of assembly sequence (OAS) of tobacco mosaic virus (TMV) is necessary for the specific encapsidation of replicating viral RNA. To this end TMV coat protein was expressed from replicating RNA constructs with or without the OASin planta. In both cases the replicating RNA was specifically encapsidated to give nucleoprotein nanorods, though the yield in the absence of the OAS was reduced to about 60% of that in its presence. Moreover, the nanorods generated in the absence of the OAS were more heterogeneous in length and contained frequent structural discontinuities. These results strongly suggest that the function of the OAS is to provide a unique site for the initiation of viral assembly, leading to a one-start helix, rather than the selection of virus RNA for packaging.