More than meets the eye? Factors that affect target selection by plant miRNAs and heterochromatic siRNAs.
More than meets the eye? Factors that affect target selection by plant miRNAs and heterochromatic siRNAs.
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DOI：
10.1016/j.pbi.2015.06.012
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发表时间：
2015-10
影响因子：
9.5
通讯作者：
Axtell MJ
中科院分区：
文献类型：
--
作者：
Wang F;Polydore S;Axtell MJ
靶向mRNA进行转录后调节的MicroRNA和靶向染色质引起DNA甲基化的异染色质siRNA构成了大多数植物标本中内源性调节小RNA库的大部分。它们都具有基于互补性将Argonaute蛋白引导至靶核酸的功能。最近关于植物miRNA-靶标相互作用的研究已经阐明了互补性的一般“规则”，同时也提供了这些规则的一些有趣的例外。此外，新出现的证据表明，除了miRNA靶互补性之外，还有几个因素影响植物miRNA功能。对于异染色质siRNA，最近的工作已经朝着全面识别潜在的靶区域取得了进展，但许多基本问题仍有待回答。
MicroRNAs, which target mRNAs for post-transcriptional regulation, and heterochromatic siRNAs, which target chromatin causing DNA methylation, make up the majority of the endogenous regulatory small RNA pool in most plant specimens. They both function to guide Argonaute proteins to targeted nucleic acids on the basis of complementarity. Recent work on plant miRNA-target interactions has clarified the general ‘rules’ of complementarity, while also providing several intriguing exceptions to these rules. In addition, emerging evidence suggests that several factors besides miRNA-target complementarity affect plant miRNA function. For heterochromatic siRNAs, recent work has made progress towards comprehensively identifying potential target regions, but numerous fundamental questions remain to be answered.