The L7/L12 stalk, a conserved feature of the prokaryotic ribosome, is attached to the large subunit through its N terminus.
The L7/L12 stalk, a conserved feature of the prokaryotic ribosome, is attached to the large subunit through its N terminus.
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DOI：
10.1016/0022-2836(81)90327-2
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发表时间：
1981-07
影响因子：
5.6
通讯作者：
D. Marquis;S. Fahnestock;E. Henderson;D. Woo;S. Schwinge;M. W. Clark;J. Lake
中科院分区：
文献类型：
--
作者：
D. Marquis;S. Fahnestock;E. Henderson;D. Woo;S. Schwinge;M. W. Clark;J. Lake
L7/L12柄是大肠杆菌核糖体大亚基的一个显著特征，是典型原核生物大亚基的一个普遍形态特征，在嗜热脂肪芽孢杆菌中，L7/L12柄由四个拷贝的核糖体蛋白B.L13组成，B.L13是大肠杆菌的功能和氨基酸序列同源物。大肠杆菌蛋白L7/L12。该蛋白质包含茎，这是通过电子显微镜的50个S亚基，已经有B.L13选择性地删除，并通过免疫电子显微镜B.L13映射。此外，已知与B.L13竞争结合B.L13缺失的50 S核心的大约由B.L13的N-末端三分之一组成的片段显示位于接近50 S亚基主体的茎区，和C端的两个B.L13的三分之一显示位于大亚基远端的柄的球状区域。分离的亚基B.L13和其它原核生物中相应的L7/L12蛋白的至少三条和可能所有四条链排列成蛋白质的N末端接触50 S亚基的体，蛋白质的C末端球状区从亚基延伸到细胞质中。
The L7/L12 stalk, a distinctive feature of the large ribosomal subunit fromEscherichia coli, is shown to be a general morphological feature associated with large subunits from representative prokaryotes.In the gram-positive thermophileBacillus stearothermophilus, the stalk consists of four copies of ribosomal proteins B.L13, the functional and amino acid sequence homologue ofE. coliproteins L7/L12. That this protein comprises the stalk is shown by electron microscopy of 50 S subunits that have had B.L13 selectively removed, and by immune electron microscopic mapping of B.L13. In addition, a fragment consisting approximately of the N-terminal one-third of B.L13, known to compete with B.L13 for binding to B.L13-depleted 50 S cores, is shown to be located in the region of the stalk proximal to the body of the 50 S subunit, and the C-terminal two-thirds of B.L13 is shown to be located in the globular region of the stalk that is distal to the large subunit.Taken together these results suggest that in the isolated subunit at least three and probably all four chains of B.L13, and of the corresponding L7/L12 proteins in other prokaryotes, are arranged with the N termini of the proteins contacting the body of the 50 S subunit and with the C-terminal globular regions of the protein extending away from the subunit into the cytoplasm.