Translational control of endogenous and recoded nuclear genes in yeast mitochondria: Regulation and membrane targeting
Translational control of endogenous and recoded nuclear genes in yeast mitochondria: Regulation and membrane targeting
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DOI：
10.1007/bf01952112
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发表时间：
1996-12-01
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影响因子：
--
通讯作者：
Fox, TD
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文献类型：
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作者：
Fox, TD
酵母（Saccharomyces cerevisiae）中的线粒体基因表达依赖于细胞核中编码的不同蛋白质对单个mRNA的翻译激活。这些核编码的mRNA特异性翻译激活剂与内膜结合，并发挥介导mRNA与线粒体核糖体之间相互作用的作用。这个复杂的系统，发现到目前为止，只有在细胞器，似乎是一个适应靶向合成的细胞膜编码的完整的膜蛋白的膜。此外，mRNA特异性翻译激活是用于响应环境条件调节至少一种线粒体基因表达的限速步骤。直接研究线粒体基因调控和靶向的线粒体编码的蛋白质在体内，现在将有可能使用合成基因插入到线粒体DNA编码可溶性报告/乘客蛋白。
Mitochondrial gene expression in yeast, Saccharomyces cerevisiae, depends on translational activation of individual mRNAs by distinct proteins encoded in the nucleus. These nuclearly coded mRNA-specific translational activators are bound to the inner membrane and function to mediate the interaction between mRNAs and mitochondrial ribosomes. This complex system, found to date only in organelles, appears to be an adaptation for targeting the synthesis of mitochondrially coded integral membrane proteins to the membrane. In addition, mRNA-specific translational activation is a rate-limiting step used to modulate expression of at least one mitochondrial gene in response to environmental conditions. Direct study of mitochondrial gene regulation and the targeting of mitochondrially coded proteins in vivo will now be possible using synthetic genes inserted into mtDNA that encode soluble reporter/passenger proteins.