RFLP MAPS OF POTATO AND THEIR ALIGNMENT WITH THE HOMOEOLOGOUS TOMATO GENOME
RFLP MAPS OF POTATO AND THEIR ALIGNMENT WITH THE HOMOEOLOGOUS TOMATO GENOME
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DOI：
10.1007/bf00229225
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发表时间：
1991-01-01
影响因子：
5.4
通讯作者：
SALAMINI, F
中科院分区：
文献类型：
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作者：
GEBHARDT, C;RITTER, E;SALAMINI, F
提出了马铃薯的 RFLP 连锁图，其中包含源自 230 个 DNA 探针的 304 个基因座和一个形态标记（块茎表皮颜色）。马铃薯的自交不亲和性位点被定位到与番茄I号染色体同源的I号染色体上。通过在马铃薯中定位染色体特异性番茄RFLP标记，反之亦然，在番茄中定位马铃薯标记，将不同的马铃薯和番茄RFLP图谱相互比对，证实了马铃薯和番茄基因组的相似性。 12 条马铃薯染色体的编号现在符合既定的番茄命名法。来自不同遗传背景的马铃薯 RFLP 图谱之间的比较揭示了标记顺序的保守性，但染色体和图谱总长度的差异。特别是，与种内杂交相比，在种间杂交中观察到图谱长度显着减少。对四个马铃薯亲本的基因组中分离比扭曲的区域的分布进行了分析。发现最显着的重组扭曲是由自交不亲和基因座引起的。
An RFLP linkage map of the potato is presented which comprises 304 loci derived from 230 DNA probes and one morphological marker (tuber skin color). The self-incompatibility locus of potato was mapped to chromosome I, which is homoeologous to tomato chromosome I. By mapping chromosome-specific tomato RFLP markers in potato and, vice versa, potato markers in tomato, the different potato and tomato RFLP maps were aligned to each other and the similarity of the potato and tomato genome was confirmed. The numbers given to the 12 potato chromosomes are now in accordance with the established tomato nomenclature. Comparisons between potato RFLP maps derived from different genetic backgrounds revealed conservation of marker order but differences in chromosome and total map length. In particular, significant reduction of map length was observed in interspecific compared to intraspecific crosses. The distribution of regions with distorted segregation ratios in the genome was analyzed for four potato parents. The most prominent distortion of recombination was found to be caused by the self-incompatibility locus.