Chitinophaga silvisoli sp. nov., isolated from forest soil
Chitinophaga silvisoli sp. nov., isolated from forest soil
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Chitinophaga silvisoli sp.
DOI：
10.1099/ijsem.0.003212
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发表时间：
2019
影响因子：
2.8
通讯作者：
Zhu Honghui
中科院分区：
文献类型：
--
作者：
Wang Chunling;Lv Yingying;Li Anzhang;Feng Guangda;Bao Gegen;Zhu Honghui
2017年9月11日，从中国广东省鼎湖山生物圈保护区（23 10 N; 112.32 E）的森林土壤中分离到一株革兰氏阴性、杆状好氧细菌，命名为K20 C18050901 T。基于16 S rRNA基因序列的系统发育分析表明，菌株K20 C18050901 T属于噬几丁虫属（Chitinophaga），与圣几丁虫NBRC 15057 T和稻几丁虫JCM 16595 T的同源性最高，分别为98.6%和96.9%。主要脂肪酸（> 10%）为异C15：0、C16：1！5c，总结特征3（C16：1！6c和/或.C16：1！7 c）和异-C17：0 3-OH。主要的呼吸醌是甲基萘醌-7。主要极性脂质为磷脂酰乙醇胺。K20 C18050901 T的基因组草图大小为8.36 Mb，DNA G+C含量为44.7 mol.%。K20 C18050901 T与C. sancti、NBRC 15057 T的抗性分别为31.40%和85.82%。根据表型、基因型和系统发育分析，菌株K20 C18050901 T代表一个新的食几丁虫属种，并命名为锡尔维土壤食几丁虫Chitinophagasilvisoli sp. nov.。模式菌株为K20 C18050901 T（=GDMCC 1.1411T=KCTC 62860 T）。
A Gram-stain-negative, rod-shaped and aerobic bacterium, designated K20C18050901T, was isolated from forest soil.collected on 11 September 2017 from Dinghushan Biosphere Reserve, Guangdong Province, PR China (23 10¢ 24¢¢ N; 112.32¢ 10¢¢ E). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain K20C18050901T belongs to the.genus Chitinophaga, and showed the highest similarities to Chitinophaga sancti NBRC 15057T (98.6 %) and Chitinophaga.oryziterrae JCM 16595T (96.9 %). The major fatty acids (>10 %) were iso-C15 : 0, C16 : 1!5c, summed feature 3 (C16 : 1!6c and/or.C16 : 1!7c) and iso-C17 : 0 3-OH. The predominant respiratory quinone was menaquinone-7. The major polar lipid was.phosphatidylethanolamine. The draft genome size of strain K20C18050901T was 8.36 Mb with a DNA G+C content of 44.7 mol.%. The digital DNA–DNA hybridization and average nucleotide identity values between strain K20C18050901T and C. sancti.NBRC 15057T were 31.40 and 85.82 %, respectively. On the basis of phenotypic, genotypic and phylogenetic analysis, strain.K20C18050901T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga silvisoli sp. nov. is.proposed. The type strain is K20C18050901T (=GDMCC 1.1411T=KCTC 62860T).